[Histonet] RE: lab math: ihc dilution
Elizabeth Chlipala
liz <@t> premierlab.com
Fri Nov 21 13:35:41 CST 2014
Sheryl
Here is how I would approach the problem. We would use the basic mathematical equation of the following (in order for this to work you need to keep the units the same)
V1 x C1 = V2 x C2
V1 - volume of stock solution needed - this is what you are solving for
C1 - concentration of stock solution - you know this its 1.56 mg/ml or 1560 ug/ml (there are 1000 ug in one mg)
V2 - volume of final solution needed - I put an arbitrary volume of 10 mls needed
C2 - concentration of final solution - you know this its 0.8ug/ml
V1 x 1560 ug/ml = 10ml x 0.8 ug/ml --- see how I made sure the units were the same I changed the mg/ml to ug/ml
V1 = .0005 mls and then I would need 9.9995 mls of diluent to make up my 0.8ug/ml concentration, you can change this to be expressed in microliters by multiplying each number by 1000.
So in uls - V1 = 0.5 uls and 9999.5 uls
from there you can determine the titer which is your total volume divided by the volume of concentrated antibody
10/.0005
That would be a 1:20,000 dilution
For a dilution such as this I would make up a stock solution of either 1:100 or 1:1000 and then dilute from there.
Now its time for the rest of you to check my math, did I do this correctly????? I went over it a few times, but you never know..............
Have a GREAT Weekend!
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com
March 10, 2014 is Histotechnology Professionals Day
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl
Sent: Friday, November 21, 2014 12:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] lab math: ihc dilution
To all you math whiz out there, please help with this math dilution.
If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use?
Thanks,
Sheryl
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
More information about the Histonet
mailing list