[Histonet] Permanent red, Fuchsin and Vector Red not exactly red...

Patsy Ruegg pruegghm <@t> hotmail.com
Thu Nov 6 17:44:23 CST 2014


agree Biocares Warp Red is very strong but still has to avoid alcohol.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com
pruegg <@t> ihctech.net


From: liz <@t> premierlab.com
To: Yves.Heremans <@t> vub.ac.be; histonet <@t> lists.utsouthwestern.edu
Date: Thu, 6 Nov 2014 13:43:09 -0700
Subject: RE: [Histonet] Permanent red, Fuchsin and Vector Red not exactly	red...
CC: 

Yves
 
Try Biocares Warp Red, you will still need to air dry the slides prior to coverslipping do not place them in alcohol, air dry, place in xylene and then mount.
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com
 
March 10, 2014 is Histotechnology Professionals Day
 
Ship to Address:
 
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
 
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Yves Heremans
Sent: Thursday, November 06, 2014 1:12 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Permanent red, Fuchsin and Vector Red not exactly red...
 
Dear Histonetters,
 
I am trying to combine a peroxidase-DAB staining (brown) with an alkaline phosphatase-staining for detection of two proteins that do not overlap.
I have already tried Dako’s Permanent Red and Fuchsin+ and Vector’s Vector Red as AP substrates but I am not getting a clear red color, rather something brownish which is not very different in color from the DAB reaction product.
Is there anything I can do to obtain a clearer red color ?
 
Yves
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