[Histonet] HELP- Cryosectioning FAT!

Balasubbramanian, Dakshnapriya dakshnapriya <@t> neo.tamu.edu
Tue May 20 10:59:16 CDT 2014

Thanks Sandra! My next step was to try that.


----- Original Message -----
From: "Sandra E. Esparza" <SEsparza <@t> seton.org>
To: "Dakshnapriya Balasubbramanian" <dakshnapriya <@t> neo.tamu.edu>
Sent: Tuesday, May 20, 2014 10:34:25 AM
Subject: RE: [Histonet] HELP- Cryosectioning FAT!

You might want to dry Liquid Nitrogen on the face of the block before you cut it.  


Sandra Esparza HT (ASCP), QIHC
Histotechnologist Mohs
Austin Dermatologic Surgery Center
512-324-7468  x84027
sesparza <@t> seton.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Balasubbramanian, Dakshnapriya
Sent: Tuesday, May 20, 2014 10:23 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HELP- Cryosectioning FAT!

Hi all,

I've been having problems with cryosectioning fatty tissue for a long time now. The section leaves a hole in the middle. I know this is probably a much discussed topic, but I've tried a lot of strategies with no luck. The tissue is of mouse origin and has micro-lesions buried inside fat. So the fat needs to be cut in order to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures. I tried rubbing the block, blade and anti-roll plate with dry ice-again,no luck. 

I rapid-freeze the tissue with OCT in LN2 and then store the block at -80C. Could I try sectioning the block directly from -80C? (never done that). 

Any other suggestions on how to tackle this?

Any advice is much appreciated!!


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