[Histonet] HELP- Cryosectioning FAT!
dakshnapriya <@t> neo.tamu.edu
Tue May 20 10:59:16 CDT 2014
Thanks Sandra! My next step was to try that.
----- Original Message -----
From: "Sandra E. Esparza" <SEsparza <@t> seton.org>
To: "Dakshnapriya Balasubbramanian" <dakshnapriya <@t> neo.tamu.edu>
Sent: Tuesday, May 20, 2014 10:34:25 AM
Subject: RE: [Histonet] HELP- Cryosectioning FAT!
You might want to dry Liquid Nitrogen on the face of the block before you cut it.
Sandra Esparza HT (ASCP), QIHC
Austin Dermatologic Surgery Center
sesparza <@t> seton.org
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Balasubbramanian, Dakshnapriya
Sent: Tuesday, May 20, 2014 10:23 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HELP- Cryosectioning FAT!
I've been having problems with cryosectioning fatty tissue for a long time now. The section leaves a hole in the middle. I know this is probably a much discussed topic, but I've tried a lot of strategies with no luck. The tissue is of mouse origin and has micro-lesions buried inside fat. So the fat needs to be cut in order to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures. I tried rubbing the block, blade and anti-roll plate with dry ice-again,no luck.
I rapid-freeze the tissue with OCT in LN2 and then store the block at -80C. Could I try sectioning the block directly from -80C? (never done that).
Any other suggestions on how to tackle this?
Any advice is much appreciated!!
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