[Histonet] BRAF (V600E) on BondMax

Cooper, Brian bcooper <@t> chla.usc.edu
Mon May 19 11:27:39 CDT 2014

Hey Histonetters,

Has anyone successfully worked up Spring Bioscience's BRAF (v600e) on the Leica Bond?  We've experimented  with different pretreatment options and serially diluted the heck out this antibody (up to 1:6,400) and we're still getting background and unexpected staining.  Our pathologists are thusfar "unconvinced." Any help would be greatly appreciated . . .

Thank you!

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357     Pager: 213-209-0184
bcooper <@t> chla.usc.edu<mailto:bcooper <@t> chla.usc.edu>

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