[Histonet] OCT Issues
patrick.lewis <@t> seattlechildrens.org
Mon May 12 16:11:53 CDT 2014
With Snap frozen tissue in OCT. If I cut sections and fix them in cold acetone for 10 minutes, (Is 2 minutes vs 10 minutes going to cause any issues?)
Is it better to dry them and then put them in DPBS or just put them straight into DPBS?
If I changed the fixative to 10% NBF would that be better for the tissues than Cold Acetone?
I am still having problems with my Frozen sections looking digested, and I am not really sure what is causing it.
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