[Histonet] MUM-1 is the word.

[Davis, Cassie]

Patrick,
      Kudos to you, you saved my sanity. It came out B-E-U-tiful. I just hope our Medical Direct likes it. Thank you, thank you, thank you!

Cassandra Davis
CDavis <@t> che-east.org
302-575-8095



________________________________
From: Patrick Laurie [foreightl <@t> gmail.com]
Sent: Thursday, March 13, 2014 11:03 AM
To: Davis, Cassie
Subject: Re: [Histonet] MUM-1

Hi Cassandra,

We run it at our lab, and our incredibly picky Hemepath is happy.  But our protocol is unusual.  We run extended CC1 and a 4 minute antibody incubation.  We are using a large B cell lymphoma for our QC, which is working very well.  If you need a QC block, we have several, I would be happy to send you one.

Thanks,

Patrick


Patrick Laurie(HT)ASCP QIHC

Histology Manager

Celligent Diagnostics, LLC

101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

Work: 704-970-3300  Cell: 704-266-0869


On Thu, Mar 13, 2014 at 9:53 AM, Davis, Cassie <CDavis <@t> che-east.org<mailto:CDavis <@t> che-east.org>> wrote:
Good morning Histonet Folks,

         I am hoping one of you will help me. I am in the process of optimizing an IHC protocol on the MUM-1 antibody on paraffin tissue for the Benchmark XTstainer and I am not thrilled with the results I am getting. I have tried the "usual adjustments" and the results are less than optimal in my opinion. I am using a normal tonsil control right now but if you have another suggestion please do not hesitate to recommend. I am praying somebody might have done this before and would be willing to share their staining protocol or tips with this.

Cassandra Davis
CDavis <@t> che-east.org<mailto:CDavis <@t> che-east.org>
302-575-8095<tel:302-575-8095>



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