[Histonet] RE: IHC antibody optimizing & validating

[Podawiltz, Thomas]

Don't even get me going. 

Tom 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Tuesday, March 25, 2014 10:28 AM
To: Davis, Cassie; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: IHC antibody optimizing & validating

Hi Cassie,

I have tried going 'requisition free" a number of times in at least three institutions. The problems I've always encountered have been with the OR nursing staff, who frankly tell us that they are too busy to have to do "one more thing" - even if they were doing the 'thing' on paper anyway. 

(Now - for a little soapbox time) I have found that while there are a lot of hard working, diligent OR nurses, far too many see patient care as done once the tissue is out.  This means that many times, insufficient information or even - God help us - incorrect information ends up on the requisitions.  But it didn't get any better electronically, in fact it was worse. Having required fields before submission helps - but only if they bother to put something useful in them.  This may not be everyone's experience, in fact I hope it is not. My problem is not with paperless (which is never really paperless) but with trusting the people who do the ordering. 

Any way, thanks for letting me have a mini-rant. It felt good!

Shalom - Bill

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Davis, Cassie
Sent: Tuesday, March 25, 2014 8:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC antibody optimizing & validating

Will you help me? I understand we are to use the known positives controls that the manufactures' recommends in the package insert when optimizing the stains, but I need to know what is your general procedure for optimizing (how many different staining protocols do you test) and validating a new antibody (how many different or "known" positive and negative tissues do you test [predictive markers I understand are 20])?

Cassandra Davis
CDavis <@t> che-east.org
302-575-8095



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