[Histonet] Re: Histonet Digest, Vol 124, Issue 25
Andrea.X.Dejager <@t> kp.org
Andrea.X.Dejager <@t> kp.org
Mon Mar 24 10:01:25 CDT 2014
We get our tattoo dyes from Royal Marker - www.royalmarker.com Have been
using them for years and are very happy with the results. These do not dry
out and cake as often.
Andrea De Jager, H.T. ASCP
Histology Manager, Regional Reference Lab
Kaiser Permanente - Colorado
Phone: 303-404-4152
Fax: 303-404-4161
email: ANDREA.X.DEJAGER <@t> KP.org
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From: histonet-request <@t> lists.utsouthwestern.edu
To: histonet <@t> lists.utsouthwestern.edu
Date: 03/23/2014 11:03 AM
Subject: Histonet Digest, Vol 124, Issue 25
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
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Today's Topics:
1. Re: specimen marking ink (E. Wayne Johnson ???)
2. Help kappa and lambda IHC on bone marrow bxs
(drmoses111 <@t> comcast.net)
----------------------------------------------------------------------
Message: 1
Date: Sun, 23 Mar 2014 07:21:48 +0800
From: "E. Wayne Johnson ???" <ewj <@t> pigsqq.org>
Subject: Re: [Histonet] specimen marking ink
To: "Davis, Cassie" <CDavis <@t> che-east.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <532E1B0C.3040607 <@t> pigsqq.org>
Content-Type: text/plain; charset=UTF-8; format=flowed
Tattooing is not only for dissidents, miscreants, the wayward,
and the Llewellyn-ites among us.
Sheep, rabbits, pigs, cattle, horses, stoats, and goats can be tattooed
also,
not so much of an expression of individuality as the need for permanent
identification.
http://www.enasco.com/c/farmandranch/Livestock%20Identification/Tattooing/
On 3:59 AM, Davis, Cassie wrote:
> Hi Histo World, as I was cutting to day I was thinking why don't we see
if we could get specimen marking ink directly from a tattoo vendor? When I
first started in histo I was told the ink we use was actually tattoo ink.
As we know as soon as somebody labels something as a "medical supply" the
price is increased. Just a cost saving thought, I mentioned it to my
immediate supervisor but she think it would be a liability issue. I
thought we could "test/validate it on skin tissue left over from a
mastectomy or extremity. Any thoughts?
>
> Cassandra Davis
> CDavis <@t> che-east.org
> 302-575-8095
>
>
>
> Confidentiality Notice:
> This e-mail, including any attachments is the
> property of Catholic Health East and is intended
> for the sole use of the intended recipient(s).
> It may contain information that is privileged and
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> not the intended recipient, please delete this message, and
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>
>
------------------------------
Message: 2
Date: Sun, 23 Mar 2014 01:38:17 +0000 (UTC)
From: drmoses111 <@t> comcast.net
Subject: [Histonet] Help kappa and lambda IHC on bone marrow bxs
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1849852922.2166556.1395538697875.JavaMail.root <@t> comcast.net>
Content-Type: text/plain; charset=utf-8
Our lab has started using Immunocal decal solution on our bone marrows.
Most of our antibodies have improved except kappa and lambda. We do not do
ISH. Kappa and lambda staining in the tonsil controls is good, The bone
marrows are now very overstrained. We use DAKO polyclonals at 1:10,000
with protease1 on the Ventana Ultra. Does anyone have a procedure?
----- Original Message -----
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From: histonet-request <@t> lists.utsouthwestern.edu
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 124, Issue 24
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When replying, please edit your Subject line so it is more specific
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Today's Topics:
  1. specimen marking ink (Davis, Cassie)
  2. Leica Reichert Jung Cryocut 1800 (King, Laurie J)
  3. RE: specimen marking ink (Wanda.Smith <@t> HCAhealthcare.com)
  4. IHC on paraffin embedded skin tissue! (Jennifer Leigh)
  5. Re: RE: specimen marking ink (David Kemler)
  6. Re: RE: specimen marking ink (Bryan Llewellyn)
  7. Re: IHC on paraffin embedded skin tissue! (C.D.G.)
----------------------------------------------------------------------
Message: 1
Date: Fri, 21 Mar 2014 13:03:35 -0400
From: "Davis, Cassie" <CDavis <@t> che-east.org>
Subject: [Histonet] specimen marking ink
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <BE7AF02009C3A74F9B8344E70F3655CBFB9B2B <@t> CHEXCMS01.one.ads.che.org>
Content-Type: text/plain; charset="iso-8859-1"
Hi Histo World, as I was cutting to day I was thinking why don't we see if
we could get specimen marking ink directly from a tattoo vendor? When I
first started in histo I was told the ink we use was actually tattoo ink.
As we know as soon as somebody labels something as a "medical supply" the
price is increased. Just a cost saving thought, I mentioned it to my
immediate supervisor but she think it would be a liability issue. I
thought we could "test/validate it on skin tissue left over from a
mastectomy or extremity. Any thoughts?
Cassandra Davis
CDavis <@t> che-east.org
302-575-8095
Confidentiality Notice:
This e-mail, including any attachments is the
property of Catholic Health East and is intended
for the sole use of the intended recipient(s).
It may contain information that is privileged and
confidential. Â Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are
not the intended recipient, please delete this message, and
reply to the sender regarding the error in a separate email.
Â
------------------------------
Message: 2
Date: Fri, 21 Mar 2014 17:45:48 +0000
From: "King, Laurie J" <king.laurie <@t> marshfieldclinic.org>
Subject: [Histonet] Leica Reichert Jung Cryocut 1800
To: "'histonet <@t> lists.utsouthwestern.edu'
        (histonet <@t> lists.utsouthwestern.edu)"
        <histonet <@t> lists.utsouthwestern.edu>
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        <7578207839F50248A7A6CD33517295EA4F13B680 <@t> MCL-EXMB03.mfldclin.org>
Content-Type: text/plain; charset="us-ascii"
Hello all,
Looking for a manual for a Leica Reichert Jung Cryocut 1800.
Laurie
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Message: 3
Date: Fri, 21 Mar 2014 14:37:59 -0500
From: <Wanda.Smith <@t> HCAhealthcare.com>
Subject: [Histonet] RE: specimen marking ink
To: <CDavis <@t> che-east.org>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <9E2D36CE2D7CBA4A94D9B22E8328A3BA27EFDDFC29 <@t> NADCWPMSGCMS03.hca.corpad.net>
       Â
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Back in the 70's (when I was six, but still a histotech!!!) my Pathologist
had gotten tattoo pigment powder in two colors and that's what we used to
mark tissue. Â Don't know where it came from and don't know where it
went!!!
Happy Friday and Happy Weekend to everyone!!!
Wanda
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC� 29406
843-847-4586
843-847-4296 fax
This email and any files transmitted with it may contain PRIVILEGED or
CONFIDENTIAL information and may be read or used only by the intended
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Davis,
Cassie
Sent: Friday, March 21, 2014 1:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] specimen marking ink
Hi Histo World, as I was cutting to day I was thinking why don't we see if
we could get specimen marking ink directly from a tattoo vendor? When I
first started in histo I was told the ink we use was actually tattoo ink.
As we know as soon as somebody labels something as a "medical supply" the
price is increased. Just a cost saving thought, I mentioned it to my
immediate supervisor but she think it would be a liability issue. I
thought we could "test/validate it on skin tissue left over from a
mastectomy or extremity. Any thoughts?
Cassandra Davis
CDavis <@t> che-east.org
302-575-8095
Confidentiality Notice:
This e-mail, including any attachments is the property of Catholic Health
East and is intended for the sole use of the intended recipient(s).
It may contain information that is privileged and confidential. Â Any
unauthorized review, use, disclosure, or distribution is prohibited. If
you are not the intended recipient, please delete this message, and reply
to the sender regarding the error in a separate email.
Â
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Fri, 21 Mar 2014 13:06:46 -0700 (PDT)
From: Jennifer Leigh <immunoqueen <@t> yahoo.com>
Subject: [Histonet] IHC on paraffin embedded skin tissue!
To: Histonet Netserver <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1395432406.35742.YahooMailNeo <@t> web120703.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
������ Histonetters-
�
���������� I have a question about mouse skin
samples----an IHC experiment has gone wrong and I am not sure why. I have
a set of paraffin embedded mouse skin samples from an inflammatory model
that were stained for F4/80. The end result is no color development after
the addition of DAB. I use the DAB tablets from Sigma using the TBS buffer
to dissolve the tablet. There is no color development, not even
non-specific staining. Any ideas what the problem might be?????� I also
performed a Proteinase K antigen retrevial step (using the ready made
solution from Dako) for 15 minutest at 37 C as I was instructed to do.
Thank you in advance for any insight!
�
Jennifer Oskins
Jennifer L. Oskins
"Until one has loved an animal, part of their soul remains
unawakened....."
------------------------------
Message: 5
Date: Fri, 21 Mar 2014 16:02:29 -0700 (PDT)
From: David Kemler <histotalk <@t> yahoo.com>
Subject: Re: [Histonet] RE: specimen marking ink
To: "Wanda.Smith <@t> HCAhealthcare.com" <Wanda.Smith <@t> HCAhealthcare.com>,
        "CDavis <@t> che-east.org" <CDavis <@t> che-east.org>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1395442949.1599.YahooMailNeo <@t> web121504.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Actually, I have a tattoo supplier four miles away from me. Several years
ago, I went to their warehouse to take a look an possibly make a purchase.
As it turned out, I had to buy a gallon of each color and it worked out to
be more than the standard tissue inks sold by the histology vendors.�
Next stop - tattoo shop. Same story. The artist would sell a small 8 oz.
bottle at cost, but it was still just a bit more than our vendors.
You can use Pelican drawing ink. It comes in several colors. It's around
five something for an ounce+, much more than Davidson or one of the
others.
Maybe someone will also chime in.
Dave�
On Friday, March 21, 2014 3:39 PM, "Wanda.Smith <@t> HCAhealthcare.com"
<Wanda.Smith <@t> HCAhealthcare.com> wrote:
Â
Back in the 70's (when I was six, but still a histotech!!!) my Pathologist
had gotten tattoo pigment powder in two colors and that's what we used to
mark tissue.� Don't know where it came from and don't know where it
went!!!
Happy Friday and Happy Weekend to everyone!!!
Wanda
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC� 29406
843-847-4586
843-847-4296 fax
This email and any files transmitted with it may contain PRIVILEGED or
CONFIDENTIAL information and may be read or used only by the intended
recipient. If you are not the intended recipient of the email or any of
its attachments, please be advised that you have received this email in
error and that any use, dissemination, distribution, forwarding, printing,
or copying of this email or any attached files is strictly prohibited. If
you have received this email in error, please immediately purge it and all
attachments and notify the sender by reply email or contact the sender at
the number listed.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Davis,
Cassie
Sent: Friday, March 21, 2014 1:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] specimen marking ink
Hi Histo World, as I was cutting to day I was thinking why don't we see if
we could get specimen marking ink directly from a tattoo vendor? When I
first started in histo I was told the ink we use was actually tattoo ink.
As we know as soon as somebody labels something as a "medical supply" the
price is increased. Just a cost saving thought, I mentioned it to my
immediate supervisor but she think it would be a liability issue. I
thought we could "test/validate it on skin tissue left over from a
mastectomy or extremity. Any thoughts?
Cassandra Davis
CDavis <@t> che-east.org
302-575-8095
Confidentiality Notice:
This e-mail, including any attachments is the property of Catholic Health
East and is intended for the sole use of the intended recipient(s).
It may contain information that is privileged and confidential.� Any
unauthorized review, use, disclosure, or distribution is prohibited. If
you are not the intended recipient, please delete this message, and reply
to the sender regarding the error in a separate email.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 6
Date: Fri, 21 Mar 2014 18:26:05 -0700
From: Bryan Llewellyn <llewllew <@t> shaw.ca>
Subject: Re: [Histonet] RE: specimen marking ink
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <532CE6AD.8050806 <@t> shaw.ca>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
You can buy tattoo ink in a bewildering array of colours from ebay quite
cheaply.
Many years ago I tried to buy tattoo ink from a tattoo parlour, but they
refused to sell it to me as they were concerned that it would be used
for rubbing into scratches and cuts for home made tattoos. I was in my
mid-50's at the time. Perhaps I looked strange with my beard and long
hair! I decided to buy the commercial tattoo ink kits from lab suppliers
for mixed colours, but used laundry blueing for most margins and kept
the tattoo inks for those occasions when it was important to know which
margin was which.
Bryan Llewellyn
David Kemler wrote:
> Actually, I have a tattoo supplier four miles away from me. Several
years ago, I went to their warehouse to take a look an possibly make a
purchase. As it turned out, I had to buy a gallon of each color and it
worked out to be more than the standard tissue inks sold by the histology
vendors.
>
> Next stop - tattoo shop. Same story. The artist would sell a small 8 oz.
bottle at cost, but it was still just a bit more than our vendors.
>
> You can use Pelican drawing ink. It comes in several colors. It's around
five something for an ounce+, much more than Davidson or one of the
others.
>
> Maybe someone will also chime in.
>
> Dave
>
>
>
> On Friday, March 21, 2014 3:39 PM, "Wanda.Smith <@t> HCAhealthcare.com"
<Wanda.Smith <@t> HCAhealthcare.com> wrote:
>
> Back in the 70's (when I was six, but still a histotech!!!) my
Pathologist had gotten tattoo pigment powder in two colors and that's what
we used to mark tissue. Â Don't know where it came from and don't know
where it went!!!
> Happy Friday and Happy Weekend to everyone!!!
> Wanda
>
> WANDA G. SMITH, HTL(ASCP)HT
> Pathology Supervisor
> TRIDENT MEDICAL CENTER
> 9330 Medical Plaza Drive
> Charleston, SC Â 29406
> 843-847-4586
> 843-847-4296 fax
>
> This email and any files transmitted with it may contain PRIVILEGED or
CONFIDENTIAL information and may be read or used only by the intended
recipient. If you are not the intended recipient of the email or any of
its attachments, please be advised that you have received this email in
error and that any use, dissemination, distribution, forwarding, printing,
or copying of this email or any attached files is strictly prohibited. If
you have received this email in error, please immediately purge it and all
attachments and notify the sender by reply email or contact the sender at
the number listed.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Davis,
Cassie
> Sent: Friday, March 21, 2014 1:04 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] specimen marking ink
>
> Hi Histo World, as I was cutting to day I was thinking why don't we see
if we could get specimen marking ink directly from a tattoo vendor? When I
first started in histo I was told the ink we use was actually tattoo ink.
As we know as soon as somebody labels something as a "medical supply" the
price is increased. Just a cost saving thought, I mentioned it to my
immediate supervisor but she think it would be a liability issue. I
thought we could "test/validate it on skin tissue left over from a
mastectomy or extremity. Any thoughts?
>
> Cassandra Davis
> CDavis <@t> che-east.org
> 302-575-8095
>
>
>
> Confidentiality Notice:
> This e-mail, including any attachments is the property of Catholic
Health East and is intended for the sole use of the intended recipient(s).
> It may contain information that is privileged and confidential. Â Any
unauthorized review, use, disclosure, or distribution is prohibited. If
you are not the intended recipient, please delete this message, and reply
to the sender regarding the error in a separate email.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 7
Date: Fri, 21 Mar 2014 23:44:20 -0300
From: "C.D.G." <latecor <@t> montevideo.com.uy>
Subject: Re: [Histonet] IHC on paraffin embedded skin tissue!
To: Histonet <@t> lists.utsouthwestern.edu
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <201403212344200234.000D0792 <@t> smtp.montevideo.com.uy>
Content-Type: text/plain; charset="ISO-8859-1"
 Jennifer be sure to add three or four drops of H2O2 to the solution.
Maybe the lack
 of oxidation of the chromogen is the cause of no color development.
 Best luck,
 Carlos.-
>������ Histonetters-
�
���������� I have a question about mouse skin
>samples----an IHC experiment has gone wrong and I am not sure why. I have
>a set of paraffin embedded mouse skin samples from an inflammatory model
>that were stained for F4/80. The end result is no color development after
>the addition of DAB. I use the DAB tablets from Sigma using the TBS
buffer
>to dissolve the tablet. There is no color development, not even
>non-specific staining. Any ideas what the problem might be?????� I also
>performed a Proteinase K antigen retrevial step (using the ready made
>solution from Dako) for 15 minutest at 37 C as I was instructed to do.
>Thank you in advance for any insight!
�
Jennifer Oskins
Jennifer L.
>Oskins
"Until one has loved an animal, part of their soul remains
>unawakened....."
>_______________________________________________
>Histonet mailing list
>
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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