[Histonet] Processing frozen brain samples

[Murphy, Valerie]

Hi,

I am new to Histonet. Here is my question.

We have brain samples that were fixed in 4% paraformaldehyde then placed in 30% sucrose (cryoprotectant) prior to freezing on dry ice. These samples have been stored at -80 for a couple of years. We want to thaw these samples then process them to make paraffin embedded blocks. What is the best way to remove the sucrose prior to processing? Should we just  rinse with PBS?



Thank you,



Valerie



Valerie Ratliff  B.Sc HTL(ASCP)

Research Assistant

Department of Oncology

Karmanos Cancer Institute

4100 John R

Detroit, MI 48201



Telephone: (313) 576 8282

Fax: (313) 576 8306

E-mail: murphyv <@t> karmanos.org<mailto:murphyv <@t> karmanos.org>



Better treatments. Better outcomes.

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