[Histonet] Decal and molecular testing

[WILLIAM DESALVO]

To get the most DNA/RNA after decal you will want to stay away from HCL. There is the Immunocal that is Formic acid based (same company offers an EDTA solution) or Milestone Medical MOL-Decal 10 that is all EDTA. The issue is both will take longer to decal than HCL solutions.
 
Fix the bone in 10% Formalin, time is dependant upon the size. Use stirring, heat, 50 degrees C, and pH 7.2-7.4. MOL-Decal 10 suggests 5-6 hrs for small biopsy bones and Immunocal 2-4 hrs.
 
Try these and test at your site. Make sure you do a validation study to confirm results and use. Good luck

William DeSalvo, BS HTL(ASCP)

 
> From: billodonnell <@t> catholichealth.net
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 11 Mar 2014 18:24:32 +0000
> Subject: [Histonet] Decal and molecular testing
> 
> Can anyone recommend a decal solution that does no damage to specimens for molecular testing - or one that has minimal damage? Thanks - Bill
> 
> 
> William (Bill) O'Donnell, HT (ASCP) QIHC 
> Senior Histologist
> Good Samaritan Hospital
> 10 East 31st Street
> Kearney, NE 68847 
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