[Histonet] looking for tips on cutting frozen fly heads

[Grantham, Andrea L - (algranth)]

I have another of those unusual projects and I'm looking for some advice of handling the drosophila flies before freezing (to fix or not to fix) and then freezing tips. Anybody have any experience with these flies?

They brought me some this morning but the results were not good. They killed the flies in 95% ETOH and strung them on a "fly collar" and put them in OCT and froze with a dry ice-alcohol slurry. Looks like ice crystals and a lot of shrinking and shattering under the scope.

Andi G




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097