[Histonet] BrDU

[Amos Brooks]

Hi,
      I use Sigma's B2531 with 1 hr pretreatment in 1N HCl at 40 deg (in a
coplin jar in my waterbath) then 15 min Trypsin (don't forget the CaCl2) at
40 deg. I detect it witb Envision+ but any mouse IgG would work fine.

Amos

Message: 15
Date: Tue, 24 Jun 2014 15:12:01 +0000
From: "Chiriboga, Luis" <Luis.Chiriboga <@t> nyumc.org>
Subject: [Histonet] BrDU
To: " (Histonet <@t> lists.utsouthwestern.edu)"
        <Histonet <@t> lists.utsouthwestern.edu>
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        <3E6798F00C9F494399E96B720ECD14292B2982 <@t> MSGWCDCPMB22.nyumc.org>
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Hi Histonetters
Can anyone recommend a good anti-BrDU antibody and protocol

Thanks  & Happy Summer!!