[Histonet] RE: rolling sections
Joana Moreira
jmoreira <@t> sidra.org
Fri Jun 13 00:57:55 CDT 2014
I agree - the sections don't need to roll. You can scrunch them and put them in the tube. I used to cut paraffin rolls / sections and manually extract DNA from them. You'll find that some roll very well some others not at all. My advice is to when you scrunch them, leave them more towards the bottom of the tube - often times when there's smaller pieces of wax in top part (and due to statics) upon opening the microtubes to start dewaxing there might be some pieces of wax coming out; same applies to smaller rolls. Also agree with the comment about increasing the thickness; however this might mean an extra step of xylene during the dewaxing process. And yes, do use and change gloves, blade and disinfect microtome sectioning area if the rolls are being used for nucleic acid extraction.
Joana
Joana Moreira
Supervisor – Anatomical Pathology
Department of Pathology
Sidra Medical & Research Center
PO Box 26999 | Doha, Qatar
Direct Line +974-4404-2036
jmoreira <@t> sidra.org | www.sidra.org
------------------------------
Message: 7
Date: Thu, 12 Jun 2014 14:54:45 +0000
From: Helen Fedor <hfedor <@t> jhmi.edu>
Subject: [Histonet] RE: rolling sections
To: 'Roberta Horner' <rjr6 <@t> psu.edu>, "Histonet
(histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BCE07FB3A86FD040969A9EC0DD3EDA0D170C7DBF <@t> JHEMTMWEX4.win.ad.jhu.edu>
Content-Type: text/plain; charset="us-ascii"
Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled.
Helen
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Thursday, June 12, 2014 10:42 AM
To: Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] rolling sections
I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws.
Roberta Horner
Animal Diagnostic Lab
Penn State University
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Thu, 12 Jun 2014 14:58:03 +0000
From: Martha Ward-Pathology <mward <@t> wakehealth.edu>
Subject: [Histonet] RE: rolling sections
To: Helen Fedor <hfedor <@t> jhmi.edu>, 'Roberta Horner' <rjr6 <@t> psu.edu>,
"Histonet (histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B2CECB1B6665A4479056478F6DE3C4AB1985D26E <@t> exchdb6.medctr.ad.wfubmc.edu>
Content-Type: text/plain; charset="iso-8859-1"
We do the same thing on our lab. It isn't necessary for them to roll....we just catch them and fold them up and put them in the tube.
?
Martha Ward, MT (ASCP) QIHC
Manager
Molecular Diagnostics Lab
Medical Center Boulevard ?\? Winston-Salem, NC 27157
p 336.716.2109 ?\? f 336.716.5890 ?
mward <@t> wakehealth.edu ?
?
?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Helen Fedor
Sent: Thursday, June 12, 2014 10:55 AM
To: 'Roberta Horner'; Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] RE: rolling sections
Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled.
Helen
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Thursday, June 12, 2014 10:42 AM
To: Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] rolling sections
I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws.
Roberta Horner
Animal Diagnostic Lab
Penn State University
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Thu, 12 Jun 2014 14:58:46 +0000
From: Bernice Frederick <b-frederick <@t> northwestern.edu>
Subject: [Histonet] RE: rolling sections
To: Martha Ward-Pathology <mward <@t> wakehealth.edu>, Helen Fedor
<hfedor <@t> jhmi.edu>, "'Roberta Horner'" <rjr6 <@t> psu.edu>, "Histonet
(histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<62C639732D3F274DACED033EBDF6ADAF2F0D02D6 <@t> evcspmbx2.ads.northwestern.edu>
Content-Type: text/plain; charset="iso-8859-1"
True.
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology
Sent: Thursday, June 12, 2014 9:58 AM
To: Helen Fedor; 'Roberta Horner'; Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] RE: rolling sections
We do the same thing on our lab. It isn't necessary for them to roll....we just catch them and fold them up and put them in the tube.
?
Martha Ward, MT (ASCP) QIHC
Manager
Molecular Diagnostics Lab
Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 mward <@t> wakehealth.edu ?
?
?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Helen Fedor
Sent: Thursday, June 12, 2014 10:55 AM
To: 'Roberta Horner'; Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] RE: rolling sections
Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled.
Helen
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Thursday, June 12, 2014 10:42 AM
To: Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] rolling sections
I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws.
Roberta Horner
Animal Diagnostic Lab
Penn State University
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Thu, 12 Jun 2014 14:59:37 +0000 (UTC)
From: Pam Marcum <mucram11 <@t> comcast.net>
Subject: Re: [Histonet] RE: rolling sections
To: Helen Fedor <hfedor <@t> jhmi.edu>
Cc: "Histonet \(histonet <@t> lists.utsouthwestern.edu\)"
<histonet <@t> lists.utsouthwestern.edu>, Roberta Horner <rjr6 <@t> psu.edu>
Message-ID:
<321366197.150096.1402585177602.JavaMail.root <@t> sz0001a.westchester.pa.mail.comcast.net>
Content-Type: text/plain; charset=utf-8
That is how we do it also.?? Since they are not really interested in the sections being flat we just pick them and put them directly into the tube.If I am really having issues I will actually warm the block with my finger to help it roll.?? If they are doing DNA or RNAase ou may need to wear glove to prevent any contamination of the blocks.??
Pam Marcum
UAMS
----- Original Message -----
From: "Helen Fedor" <hfedor <@t> jhmi.edu>
To: "Roberta Horner" <rjr6 <@t> psu.edu>, "Histonet (histonet <@t> lists.utsouthwestern.edu)" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, June 12, 2014 9:54:45 AM
Subject: [Histonet] RE: rolling sections
Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled.
Helen
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Thursday, June 12, 2014 10:42 AM
To: Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] rolling sections
I have some researchers that want to do PCR. ??They want 10 - 10u sections in a micro-centrifuge tube. ??The only way to get the sections in the tube is for the sections to roll. ??How do you get sections to roll when you want them to roll? ??I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. ??When I want a thick ribbon it will roll, darn that Murphy and his laws.
Roberta Horner
Animal Diagnostic Lab
Penn State University
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Thu, 12 Jun 2014 15:08:22 +0000
From: "Truscott, Tom" <ttruscot <@t> vetmed.wsu.edu>
Subject: RE: [Histonet] RE: rolling sections
To: Pam Marcum <mucram11 <@t> comcast.net>, Helen Fedor <hfedor <@t> jhmi.edu>
Cc: "Histonet \(histonet <@t> lists.utsouthwestern.edu\)"
<histonet <@t> lists.utsouthwestern.edu>, Roberta Horner <rjr6 <@t> psu.edu>
Message-ID:
<9EF5279EBDFE6E4FB6605E8F183A00278ED10667 <@t> CVM76.vetmed.wsu.edu>
Content-Type: text/plain; charset="utf-8"
Hi Roberta, Check with those requesting to see if you can cut the equilivant but with thicker sections that will roll- like 5 20's or 2 50's. The rolls are lots easier to get into the tubes. Tom T
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pam Marcum
Sent: Thursday, June 12, 2014 8:00 AM
To: Helen Fedor
Cc: Histonet (histonet <@t> lists.utsouthwestern.edu); Roberta Horner
Subject: Re: [Histonet] RE: rolling sections
That is how we do it also.?? Since they are not really interested in the sections being flat we just pick them and put them directly into the tube.If I am really having issues I will actually warm the block with my finger to help it roll.?? If they are doing DNA or RNAase ou may need to wear glove to prevent any contamination of the blocks.??
Pam Marcum
UAMS
----- Original Message -----
From: "Helen Fedor" <hfedor <@t> jhmi.edu>
To: "Roberta Horner" <rjr6 <@t> psu.edu>, "Histonet (histonet <@t> lists.utsouthwestern.edu)" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, June 12, 2014 9:54:45 AM
Subject: [Histonet] RE: rolling sections
Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled.
Helen
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Thursday, June 12, 2014 10:42 AM
To: Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] rolling sections
I have some researchers that want to do PCR. ??They want 10 - 10u sections in a micro-centrifuge tube. ??The only way to get the sections in the tube is for the sections to roll. ??How do you get sections to roll when you want them to roll? ??I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. ??When I want a thick ribbon it will roll, darn that Murphy and his laws.
Roberta Horner
Animal Diagnostic Lab
Penn State University
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Thu, 12 Jun 2014 11:08:10 -0500
From: anita <azdudley <@t> hotmail.com>
Subject: [Histonet] Processors again
To: "Histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <COL131-W54C1BA9338C2B721FF6528D12A0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Sorry to bring this up again but wondering if anyone has used the Milestone Logos One? I have info on the Excelsior, and we curantly have a VIP. Just wanting to get ingo and thoughts on them all.
Thanks a bunch!!!
Have a great day!!!
Anita Dudley
Providence Hospital
Mobile, Al.
------------------------------
Message: 13
Date: Thu, 12 Jun 2014 11:08:07 -0500
From: "Del Phillips" <dphillips <@t> vetmed.lsu.edu>
Subject: [Histonet] Please remove me
To: <histonet <@t> lists.utsouthwestern.edu>,
<histonet-request <@t> lists.utsouthwestern.edu>, "J Hawke"
<jhawke1 <@t> lsu.edu>
Message-ID: <003801cf8658$7f91a850$7eb4f8f0$@vetmed.lsu.edu>
Content-Type: text/plain; charset="us-ascii"
Please remove me
------------------------------
Message: 14
Date: Thu, 12 Jun 2014 12:13:20 -0400
From: Ronda Mire <rmire <@t> cvpath.org>
Subject: Re: [Histonet] Processors again
To: anita <azdudley <@t> hotmail.com>
Cc: "Histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <8E38A568-1242-4696-8658-FEED69688AD5 <@t> cvpath.org>
Content-Type: text/plain; charset=iso-8859-1
I do not like the Excelsior, not very user friendly. The VIP is good but my favorite is the Leica Peloris
On Jun 12, 2014, at 12:08 PM, anita <azdudley <@t> hotmail.com> wrote:
> Sorry to bring this up again but wondering if anyone has used the Milestone Logos One? I have info on the Excelsior, and we curantly have a VIP. Just wanting to get ingo and thoughts on them all.
>
>
>
> Thanks a bunch!!!
>
>
>
> Have a great day!!!
>
>
>
> Anita Dudley
>
> Providence Hospital
>
> Mobile, Al.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Thu, 12 Jun 2014 16:20:06 +0000
From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
Subject: RE: [Histonet] Processors again
To: Ronda Mire <rmire <@t> cvpath.org>, anita <azdudley <@t> hotmail.com>
Cc: "Histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<41D3A1AF6FEF0643BDC89E0516A6EA32012403B041 <@t> Mail2Node2.ad.uams.edu>
Content-Type: text/plain; charset="us-ascii"
We have 4 Excelsiors and love them. We also have one Leica ASP300 and it is a great workhorse for us. I think it just depends on your lab and what you like or what serves you best.
Pam Marcum
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ronda Mire
Sent: Thursday, June 12, 2014 11:13 AM
To: anita
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Processors again
I do not like the Excelsior, not very user friendly. The VIP is good but my favorite is the Leica Peloris On Jun 12, 2014, at 12:08 PM, anita <azdudley <@t> hotmail.com> wrote:
> Sorry to bring this up again but wondering if anyone has used the Milestone Logos One? I have info on the Excelsior, and we curantly have a VIP. Just wanting to get ingo and thoughts on them all.
>
>
>
> Thanks a bunch!!!
>
>
>
> Have a great day!!!
>
>
>
> Anita Dudley
>
> Providence Hospital
>
> Mobile, Al.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 16
Date: Thu, 12 Jun 2014 16:20:32 +0000
From: "Weems, Joyce K." <Joyce.Weems <@t> emoryhealthcare.org>
Subject: RE: [Histonet] Please remove me
To: "'Del Phillips'" <dphillips <@t> vetmed.lsu.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>,
"histonet-request <@t> lists.utsouthwestern.edu"
<histonet-request <@t> lists.utsouthwestern.edu>, J Hawke <jhawke1 <@t> lsu.edu>
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<E3A4EBD57A691646BCCED4AA5911A030CB5F33D8 <@t> e14mbx12n.Enterprise.emory.net>
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Hi Don,
You must do that yourself at http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Thanks and best wishes!
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems <@t> emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Del Phillips
Sent: Thursday, June 12, 2014 12:08 PM
To: histonet <@t> lists.utsouthwestern.edu; histonet-request <@t> lists.utsouthwestern.edu; J Hawke
Subject: [Histonet] Please remove me
Please remove me
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Message: 17
Date: Thu, 12 Jun 2014 12:21:42 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: Re: [Histonet] rolling sections
To: "Roberta Horner" <rjr6 <@t> psu.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9648A1710654CB587C77A28B8533CD5 <@t> HP2010>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Try changing the angle of the knife blade, so that the clearance angle
(angle between the knife blade and the block face) is larger. In other
words, tip the top of the blade towards the block more. If there are numbers
on the side of the knife holder, you want to move it to a larger number
(like from 5 to 10). Before sectioning, remember to move the block holder
towards you, since the blade will now be closer to the block, and you don't
want to ker-chunk the block.
And remember, when done, to return the clearance angle back to it's usual
location, so you aren't curling all your ribbons. So look at the number it
is usually set at, or, if there is no number, make some marks on the side of
the knife holder, that you can line up again.
Peggy A. Wenk, HTL(ASCP)SLS
-----Original Message-----
From: Roberta Horner
Sent: Thursday, June 12, 2014 10:42 AM
To: Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] rolling sections
I have some researchers that want to do PCR. They want 10 - 10u sections in
a micro-centrifuge tube. The only way to get the sections in the tube is
for the sections to roll. How do you get sections to roll when you want
them to roll? I've tried room temperature, on ice, brand new sharp blade,
dull blade and I can still get some really nice ribbons. When I want a
thick ribbon it will roll, darn that Murphy and his laws.
Roberta Horner
Animal Diagnostic Lab
Penn State University
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Thu, 12 Jun 2014 16:45:11 +0000
From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
Subject: [Histonet] Vantange/ block printer
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <5A33C952BB67F4468AF1F36D739212BC01124EC5DE <@t> JERRY.Gia.com>
Content-Type: text/plain; charset="utf-8"
For those of you who have Vantage, which block printer do you use? I've heard Leica has one with 3-6 hoppers that can print the 2D barcode on it pretty fast. I currently have the TBS block printer, but I'm told it can print the bar code, but very slow...Any suggestions?
Thanks!
------------------------------
Message: 19
Date: Thu, 12 Jun 2014 16:48:44 +0000 (UTC)
From: Pam Marcum <mucram11 <@t> comcast.net>
Subject: Re: [Histonet] Vantange/ block printer
To: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2090207285.151194.1402591724456.JavaMail.root <@t> sz0001a.westchester.pa.mail.comcast.net>
Content-Type: text/plain; charset=utf-8
Thermo also has a cassette printer that works well.?? We have t he cassette printer (6 hoppers per unit) and slide writers that work for us.??
Pam Marcum
----- Original Message -----
From: "Amber McKenzie" <amber.mckenzie <@t> gastrodocs.net>
Cc: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, June 12, 2014 11:45:11 AM
Subject: [Histonet] Vantange/ block printer
For those of you who have Vantage, which block printer do you use? ??I've heard Leica has one with 3-6 hoppers that can print the 2D barcode on it pretty fast. ??I currently have the TBS block printer, but I'm told it can print the bar code, but very slow...Any suggestions?
Thanks!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 20
Date: Thu, 12 Jun 2014 16:59:21 +0000
From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: [Histonet] RE: Vantange/ block printer
To: "'Amber McKenzie'" <amber.mckenzie <@t> gastrodocs.net>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<761E2B5697F795489C8710BCC72141FF36787853 <@t> ex07.net.ucsf.edu>
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Amber we got the Leica inkjet printer (6 actually). The 2D code reads very well. It prints at 5-7 seconds per cassette (depends on how much text you cram on there!). It is about the fastest printer out there. Data General is about the same speed, if you print with a reverse image ( black background- it uses the resin-coated cassette and laser ablation to form the image), slower with positive image (10-12 seconds - has to remove more of the black resin) . The TBS is slower because it uses a stylus to etch the plastic. Thermo printmate is also much slower (20 seconds per cassette in our tests). A surprise to me was that the cured ink from the Leica is also the most durable of all we tested. Very difficult to scratch off. The only drawback is that it is HUGE.
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Thursday, June 12, 2014 9:45 AM
Cc: Histonet
Subject: [Histonet] Vantange/ block printer
For those of you who have Vantage, which block printer do you use? I've heard Leica has one with 3-6 hoppers that can print the 2D barcode on it pretty fast. I currently have the TBS block printer, but I'm told it can print the bar code, but very slow...Any suggestions?
Thanks!
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End of Histonet Digest, Vol 127, Issue 17
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