[Histonet] RE: rolling sections

[Bernice Frederick]

True.

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology
Sent: Thursday, June 12, 2014 9:58 AM
To: Helen Fedor; 'Roberta Horner'; Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] RE: rolling sections

We do the same thing on our lab.   It isn't necessary for them to roll....we just catch them and fold them up and  put them in the tube.
 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890 mward <@t> wakehealth.edu  
 
 



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Helen Fedor
Sent: Thursday, June 12, 2014 10:55 AM
To: 'Roberta Horner'; Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] RE: rolling sections

Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled.

Helen

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Thursday, June 12, 2014 10:42 AM
To: Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] rolling sections

I have some researchers that want to do PCR.  They want 10 - 10u sections in a micro-centrifuge tube.  The only way to get the sections in the tube is for the sections to roll.  How do you get sections to roll when you want them to roll?  I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons.  When I want a thick ribbon it will roll, darn that Murphy and his laws.
Roberta Horner
Animal Diagnostic Lab
Penn State University
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