[Histonet] RE: rolling sections

[Helen Fedor]

Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled.

Helen

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roberta Horner
Sent: Thursday, June 12, 2014 10:42 AM
To: Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] rolling sections

I have some researchers that want to do PCR.  They want 10 - 10u sections in a micro-centrifuge tube.  The only way to get the sections in the tube is for the sections to roll.  How do you get sections to roll when you want them to roll?  I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons.  When I want a thick ribbon it will roll, darn that Murphy and his laws.
Roberta Horner
Animal Diagnostic Lab
Penn State University
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