[Histonet] Processor problem

Sat Jun 7 15:08:04 CDT 2014

Hi Mike,
Edit the 'dummy' run and make station 3 with '0' time.
It is time based and has a safety mechanism. That's why it pumps the previous station. Then test again.

A couple things may be an issue and need to be tested.
1. A hole, leak in the line of station 3.
2. Volume in reservoir bottle is too high
3. Kink in tube
4. Valve 'sticking'. Do the verify 7,8 test to hear the valve click in and out.
A drop of xylene will fix this. Do this under supervision. 
5. Check valve pressure and vacuum. View from behind and see the guage.
6. Clog in line 3. Do a hot water flush. 2-3 times.

Regards
Mitchell Wan
0418 745 750

P.O. BOX 2200,
Runcorn, 
Brisbane,
QLD 4113

-------- Original message --------
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Date:08/06/2014  3:03 AM  (GMT+10:00) 
To: histonet <@t> lists.utsouthwestern.edu 
Subject: Histonet Digest, Vol 127, Issue 10 

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Today's Topics:

   1. Processor problem (Mike Tighe)
   2. RE: Processor malfunction - tissues not submerged ~ 9 hours
      (Shirley A. Powell)
   3. paraffin dispenser (Davis, Cassie)
   4. Re: Processor malfunction - tissues not submerged ~ 9 hours
      (jsjurczak <@t> comcast.net)
   5. Re: Processor problem (Rene J Buesa)
   6. RE: Processor problem (Denice Stiner)
   7. VIP3000 reagent bottle (Mitchell Wan (Wanpost))

----------------------------------------------------------------------

Message: 1
Date: Fri, 6 Jun 2014 17:52:27 +0000
From: Mike Tighe <mtighe <@t> trudeauinstitute.org>
Subject: [Histonet] Processor problem
To: "histonet <@t> lists.utsouthwestern.edu
(histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1402077076740.7582 <@t> trudeauinstitute.org>
Content-Type: text/plain; charset="iso-8859-1"

I am having a problem with our tissue processor (Tissue Tek 3000). During processing the first reagent (NBF) gets pumped into the retort without any problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 70%) however, gets about half way through pumping into retort and stops. After that it reverses and pumps out reagent three, then pumps in half of reagent 2. Pumps out the half of reagent 2 and pumps in half of reagent 1. This is followed by a low fluid warning.

I have removed and reinserted the reagent reservoirs and suction tube. The tubing is not caught in the handle and seems to be fine. I have created a short (5min) automatic run with 5 minutes per station (no samples) and this problem repeats itself.

Has anybody had this problem?

Thanks for any help!

Mike

------------------------------

Message: 2
Date: Fri, 6 Jun 2014 13:53:07 -0400
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: RE: [Histonet] Processor malfunction - tissues not submerged
~ 9 hours
To: "O'Donnell, Bill" <billodonnell <@t> catholichealth.net>, "Conway,
Carla"	<cmconway <@t> usgs.gov>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9BF995BC0E47744E9673A41486E24EE25BF92F5E81 <@t> MERCERMAIL.MercerU.local>
Content-Type: text/plain; charset="utf-8"

Oh where is the like button.  :)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Friday, June 06, 2014 12:22 PM
To: Conway, Carla; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

Not since 1978. (Sorry - couldn't resist - Happy Friday!)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Friday, June 06, 2014 9:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours

Hello everyone,

When it is working correctly, our tissue processor moves a basket of tissues through 10 reagent containers. Last night it malfunctioned and suspended the tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this morning. I will process them next week. Has this happened to anyone else and what tissue artifacts can I expect?

Thanks very much,

Carla

Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-2042
Fax: 206-526-6654
E-mail: cmconway <@t> usgs.gov
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------------------------------

Message: 3
Date: Fri, 6 Jun 2014 14:31:45 -0400
From: "Davis, Cassie" <CDavis <@t> che-east.org>
Subject: [Histonet] paraffin dispenser
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BE7AF02009C3A74F9B8344E70F3655CB0AB48EF737 <@t> CHEXCMS01.one.ads.che.org>
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I recommend a slide incubator and a metal cup or two. The paraffin dispenser we purchased seem to be a relicensed commercial coffee pot. The spigot keeps clogging because they did not design it in a way that it would stay warm so, the paraffin cools in it and clogs. 

Cassie D.

Michele" <MMargiotta <@t> bmhmc.org>
Subject: [Histonet] paraffin dispenser

Message: 1
Date: Fri, 6 Jun 2014 16:04:55 +0000
From: "Margiotta-Watz, Michele" <MMargiotta <@t> bmhmc.org>
Subject: [Histonet] paraffin dispenser
To: "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <230D0B9EC57D7A45A7A186C6AB4C7ABC2A5E2C87 <@t> BMH-EXCHANGE-01.BMHMC.ORG>
Content-Type: text/plain; charset="us-ascii"

Hi All,
Anyone have a good recommendation for a reliable paraffin dispenser? The spigot keeps clogging on the one we have now.

Thanks,

Michele Margiotta-Watz
Histology Supervisor
BMHMC
101 Hospital Rd.
Patchogue, NY 11772
631-654-7192

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------------------------------

Message: 4
Date: Fri, 6 Jun 2014 18:43:13 +0000 (UTC)
From: jsjurczak <@t> comcast.net
Subject: Re: [Histonet] Processor malfunction - tissues not submerged
~ 9 hours
To: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<2129120167.2182515.1402080193013.JavaMail.root <@t> comcast.net>
Content-Type: text/plain; charset=utf-8

Yet another reason to go microwave. This can't happen. 

----- Original Message -----

From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu> 
To: "Bill O'Donnell" <billodonnell <@t> catholichealth.net>, "Carla Conway" <cmconway <@t> usgs.gov>, histonet <@t> lists.utsouthwestern.edu 
Sent: Friday, June 6, 2014 12:53:07 PM 
Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours 

Oh where is the like button. :) 

-----Original Message----- 
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill 
Sent: Friday, June 06, 2014 12:22 PM 
To: Conway, Carla; histonet <@t> lists.utsouthwestern.edu 
Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours 

Not since 1978. (Sorry - couldn't resist - Happy Friday!) 

-----Original Message----- 
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Conway, Carla 
Sent: Friday, June 06, 2014 9:15 AM 
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours 

Hello everyone, 

When it is working correctly, our tissue processor moves a basket of tissues through 10 reagent containers. Last night it malfunctioned and suspended the tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this morning. I will process them next week. Has this happened to anyone else and what tissue artifacts can I expect? 

Thanks very much, 

Carla 

Carla Conway 
Histology Technician 
Western Fisheries Research Center, USGS 
6505 N.E. 65th Street 
Seattle, WA 98115-5016 USA 
Phone: 206-526-2042 
Fax: 206-526-6654 
E-mail: cmconway <@t> usgs.gov 
_______________________________________________ 
Histonet mailing list 
Histonet <@t> lists.utsouthwestern.edu 
https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=%2BfCU3trcgbZTxcTVVwV9PBYwA4sdkdeS3%2B8QsbqJBIg%3D%0A&s=f70156bac2d9c01ccd25a40b1c3fd9f124a0fe93c1fba0c52d4e1ef48da7a09e 

This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. 

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------------------------------

Message: 5
Date: Fri, 6 Jun 2014 13:56:15 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Processor problem
To: Mike Tighe <mtighe <@t> trudeauinstitute.org>,
"histonet <@t> lists.utsouthwestern.edu
\(histonet <@t> lists.utsouthwestern.edu\)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1402088175.66031.YahooMailNeo <@t> web120405.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

My advise is to call Sakura and schedule a maintenance visit. The rotary valve may be "acting up".
Ren� J. 

On Friday, June 6, 2014 1:53 PM, Mike Tighe <mtighe <@t> trudeauinstitute.org> wrote:

I am having a problem with our tissue processor (Tissue Tek 3000). During processing the first reagent (NBF) gets pumped into the retort without any problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 70%) however, gets about half way through pumping into retort and stops. After that it reverses and pumps out reagent three, then pumps in half of reagent 2. Pumps out the half of reagent 2 and pumps in half of reagent 1. This is followed by a low fluid warning.

I have removed and reinserted the reagent reservoirs and suction tube. The tubing is not caught in the handle and seems to be fine. I have created a short (5min) automatic run with 5 minutes per station (no samples) and this problem repeats itself.

Has anybody had this problem?

Thanks for any help!

Mike
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 6
Date: Fri, 6 Jun 2014 14:19:53 -0700
From: Denice Stiner <Denice.Stiner <@t> nahealth.com>
Subject: RE: [Histonet] Processor problem
To: Rene J Buesa <rjbuesa <@t> yahoo.com>, Mike Tighe
<mtighe <@t> trudeauinstitute.org>, "histonet <@t> lists.utsouthwestern.edu
(histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BDDAB7EA6EC20647937151D5FCFDFAA72D0B74BCB5 <@t> fmexmbx02.nah1.com>
Content-Type: text/plain; charset="iso-8859-1"

I do not have a Tissue Tek 3000 but it sounds like maybe there is a sensor that needs wiped clean.  Clearly it's not ready the fluid level in station 3 and returning it back to 1 for safe keeping.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, June 06, 2014 1:56 PM
To: Mike Tighe; histonet <@t> lists.utsouthwestern.edu (histonet <@t> lists.utsouthwestern.edu)
Subject: Re: [Histonet] Processor problem

My advise is to call Sakura and schedule a maintenance visit. The rotary valve may be "acting up".
Ren� J. 

On Friday, June 6, 2014 1:53 PM, Mike Tighe <mtighe <@t> trudeauinstitute.org> wrote:

I am having a problem with our tissue processor (Tissue Tek 3000). During processing the first reagent (NBF) gets pumped into the retort without any problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 70%) however, gets about half way through pumping into retort and stops. After that it reverses and pumps out reagent three, then pumps in half of reagent 2. Pumps out the half of reagent 2 and pumps in half of reagent 1. This is followed by a low fluid warning.

I have removed and reinserted the reagent reservoirs and suction tube. The tubing is not caught in the handle and seems to be fine. I have created a short (5min) automatic run with 5 minutes per station (no samples) and this problem repeats itself.

Has anybody had this problem?

Thanks for any help!

Mike
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 7
Date: Sat, 07 Jun 2014 18:21:43 +1000
From: "Mitchell Wan (Wanpost)" <mitchell <@t> wanpost.com>
Subject: [Histonet] VIP3000 reagent bottle
To: HistoNET <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <nk5n0emi9ojqaj53ety2inf4.1402129262956 <@t> email.android.com>
Content-Type: text/plain; charset=utf-8

Hi All,
Does anyone know where I can purchase some spare vip3000 reagent bottles?

Regards
Mitchell Wan
0418 745 750

P.O. BOX 2200,
Runcorn,��
Brisbane,
QLD 4113

------------------------------

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End of Histonet Digest, Vol 127, Issue 10
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