[Histonet] Practice tissue?
Bryan Llewellyn
llewllew <@t> shaw.ca
Tue Jun 3 18:17:02 CDT 2014
Go to a butcher and ask what fresh offal they have. That should be much
the same as autopsy tissue. In addition, heart, liver, kidney and steak
from a supermarket should be OK. If you can get some, a fresh placenta
is good for H&E staining with a good mixture of elements, human or
animal makes no difference. Check with a local veterinarian or animal
pound. Sad to say that many unwanted animals are euthanized and their
tissue might be available. If you are close to a slaughterhouse, I
always found them to be very accommodating for tissue requests from pigs
and so on. Rabbit breeders will sell a live rabbit for a reasonable
amount, if you can bring yourself to kill it. They may even kill it for
you if they farm rabbits for meat.
Bryan Llewellyn
David Waugh wrote:
> Hello histonet, I was a member a number of years ago, but had gotten out of doing much histology (I was never a pro) for awhile…. but I seem to be back at it.
> I was wondering if people might have some ideas on a good “practice tissue”, one that I could embed, section, and stain (H&E) to both practice my sectioning, and check my staining protocol, etc. I really need to be sectioning some decalcified teeth, and have had some luck, but I would really like to do some more practice on an “easy to section tissue” (other than just a blank block of paraffin) that I can also test my staining on. I have accesses to a tissue processor. Is there anything I can just pick up at the grocery store? Thanks, David
> David A. Waugh, Ph.D.
> Research Assistant
> Department of Anatomy and Neurobiology
> NEOMED
> 4209 State Route 44
> Rootstown, OH 44272
>
>
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