[Histonet] Schiff : room temp or not

[Sanders, Jeanine (CDC/OID/NCEZID)]

We purchase ours from Newcomer and it is stable at room temp. I check it periodically and have never had it go weak before the expiration date. That is one of the reasons I began using it was it was always ready to use. Newcomer does note that it is sensitive to humidity and to keep tightly closed. We wrap lab tape around the lid to aid with that.


Jeanine H. Sanders


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of j.rowaihi
Sent: Thursday, July 31, 2014 1:19 PM
To: Rene J Buesa; Michael Backhus; Hans B Snyder; histonet <@t> lists.utsouthwestern.edu
Subject: رد: Re: [Histonet] Schiff : room temp or not

I am totally agree with you Rene 
I also experienced the same problem with CAP regarding Schiff's reagent storing.


Regards

Jamal Rowaihi
Laboratory supervisor
Al Borg Medical Laboratories

*Sent from my cell phoner*

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من: Rene J Buesa <rjbuesa <@t> yahoo.com> 
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إلى: Michael Backhus <mikeykkk_3 <@t> live.com>,Hans B Snyder <hans <@t> histologistics.com>,histonet <@t> lists.utsouthwestern.edu 
الموضوع: Re: [Histonet] Schiff : room temp or not 
 
 This is another example of the ignorance level of some Joint Commission "experts" that really do not know what they are talking about.
The Schiff reagent (the "hay yellow" solution of Basic Fucsin) is temperature sensitive and HAS to be stored refrigerated or it will lose its reactivity in just a few days. 
René J. 


On Wednesday, July 30, 2014 8:34 PM, Michael Backhus <mikeykkk_3 <@t> live.com> wrote:
  



         Joint Commission told us we could not keep it in the refrigerator because the bottle said to store it at room temperature. We called the manufacturer and they faxed us a paper stating we could keep it in the fridge. However It wasn't easy to get the paper because the manufacturer prefers the shelf live to be shorter so we buy more. 
Mike
Sent from my iPhone

> On Jul 30, 2014, at 8:14 PM, Hans B Snyder <hans <@t> histologistics.com> wrote:
> 
> Hello Amber,
> 
> That depends on your protocol. Which protocol are you using and what
> does your protocol say to use the schiffs at?  Consider that schiffs
> is usually stored at 4C in brown or opaque bottles.  This suggests it
> is light sensitive.  Our protocol say to use the schiffs at 37C for 15
> minutes but I have used it at 22C for 30 minutes and at 60C for 10
> minutes with consistent results.  We have found that using it at 60C
> reduces it's shelf life considerably and do not suggest it unless you
> are familiar with doing so.
> 
> Good luck
> Hans B Snyder
> Histologistics
> 60 Prescott Street
> Worcester, MA 01605
> 508-308-7800
> hans <@t> histologistics.com
> 
> 
> On Wed, Jul 30, 2014 at 4:13 PM, Amber McKenzie
> <amber.mckenzie <@t> gastrodocs.net> wrote:
>> 
>> When staining PAS's, does the Schiff need to be room temp or does it matter?  Just curious about when to add it to our Sakura stainer.  Should we put the schiff's on at the beginning of our shift so it's room temp when we need it or when should add it when we add PAS slides to it that need to be stained? Thanks!
>> 
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