AW: [Histonet] Fwd: Embedding skin
gu.lang <@t> gmx.at
Thu Jul 31 05:24:59 CDT 2014
As a first information, we use sliding microtomes with the knife set in an
angel (called declination).
We embed straight, because areas of same concistancy should be positioned in
one line and in the cutting direction.
Areas of more rigidity compress the smoother ones, if they are shifted in an
If you have problems with the orientation of the tissue in the block, just
turn the blockholder.
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Sanjeet
Gesendet: Donnerstag, 31. Juli 2014 03:46
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Fwd: Embedding skin
Sent from my iPhone
Begin forwarded message:
> From: Sanjeet <asanjeet <@t> yahoo.com>
> Date: July 30, 2014 at 9:27:46 PM EDT
> To: "histonet-request <@t> lists.utsouthwestern.edu"
<histonet-request <@t> lists.utsouthwestern.edu>
> Subject: Embedding skin
> Hi Histo techs
> Need some info on the correct orientation of skin tissue. Punch/ ellipse.
Does anyone have a literature on this topic. I am used to embed large skin
in an angle, the skin being on top.
> Currently the place where I work have different protocol, the skin is
embedded straight, the epidermis being right angled to the mold.
> I find the section difficult to cut when the skin is embedded straight ,
the section are compressed and more chances scoring along the section.
> Sent from my iPhone
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