bburnett <@t> CapeCodHealth.org
Fri Jul 25 09:35:45 CDT 2014
I was wondering what stain protocols are being used for Calponin to minimize
cross-reactivity with myofibroblasts?
We are using a Dako concentrate antibody. I recently re-optimized the
stain using a 1:100 dilution and changed the pre-treatment from low pH
HIER, to Protease only. This helped a lot with cross-reactivity with myofibroblasts
that we were seeing, however we are still seeing some non-specific staining
from time to time. Any information would be greatly appreciated!
Cape Cod Hospital
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