[Histonet] Calponin

Burnett, Brandy bburnett <@t> CapeCodHealth.org
Fri Jul 25 09:35:45 CDT 2014

Hi All,

I was wondering what stain protocols are being used for Calponin to minimize

cross-reactivity with myofibroblasts?

We are using a Dako concentrate antibody. I recently re-optimized the

stain using a 1:100 dilution and changed the pre-treatment from low pH

HIER, to Protease only. This helped a lot with cross-reactivity with myofibroblasts

that we were seeing, however we are still seeing some non-specific staining

from time to time. Any information would be greatly appreciated!


Brandy Burnett

Cape Cod Hospital

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