[Histonet] RE: Xylene Substitute Protocols

[Shannon Kleiner]

Richard Allen mounting media REF 4111.
 


>>> Shannon Kleiner 7/24/2014 12:59 PM >>>
Formula 83 in first three stations and last four stations on the Leica XL stainer.
 


>>> "Wineman, Terra" <Terra.Wineman <@t> novusint.com> 7/24/2014 12:06 PM >>>
We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based mounting type

Terra Wineman, HTL(ASCP)cm
Novus International

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hilliard, Dawud Deshawn
Sent: Thursday, July 24, 2014 11:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitute Protocols

Hello all,

Would anyone mind sharing their xylene substitute protocols for their automatic staining, to include the mountant type for coverslipping? We have tried a few, but without much success. We are currently trying Leica's Sub-X, also without much success.

I greatly appreciate the insight!

Dawud Hilliard
MPM, HTL (ASCP) QIHC
Facility Director
Animal Histopathology Core Lab
Lineberger Comprehensive Cancer Center
University of North Carolina - Chapel Hill Chapel Hill, North Carolina 27599
(919) 966-3653

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