[Histonet] Xylene Substitute Protocols

[Hilliard, Dawud Deshawn]

Hello all,

Would anyone mind sharing their xylene substitute protocols for their automatic staining, to include the mountant type for coverslipping? We have tried a few, but without much success. We are currently trying Leica's Sub-X, also without much success.

I greatly appreciate the insight!

Dawud Hilliard
MPM, HTL (ASCP) QIHC
Facility Director
Animal Histopathology Core Lab
Lineberger Comprehensive Cancer Center
University of North Carolina - Chapel Hill
Chapel Hill, North Carolina 27599
(919) 966-3653