[Histonet] Brain tissue coming off slide

Rene J Buesa rjbuesa <@t> yahoo.com
Sat Jul 19 09:31:30 CDT 2014

Generally speaking, brain tissue is likely to fell off if:
1- the section is too thick or "wavy", or if
2- the tissue has some infiltration problems and brain is one tissue difficult to process/infiltrate correctly.
Besides any problems with temp. during HIER or the type of adhesive or type of slide you use, please take a look at your protocol especially if the sections fall off during HIER and not during routine staining.
René J.  

On Friday, July 18, 2014 10:44 AM, "Murphy, Valerie" <murphyv <@t> karmanos.org> wrote:

We are staining human FFPE brain sections for Her 2 Neu and we're having a problem with tissue coming off the slide during the HIER step.
We are currently using Vectabond coated plus slides and using a decloaking chamber for HIER.
Are there any other steps we can take to prevent  tissue coming off the slide?

Thank you,

Valerie Ratliff
Karmanos Cancer Institute

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