[Histonet] Brain tissue coming off slide

[Rene J Buesa]

Generally speaking, brain tissue is likely to fell off if:
1- the section is too thick or "wavy", or if
2- the tissue has some infiltration problems and brain is one tissue difficult to process/infiltrate correctly.
Besides any problems with temp. during HIER or the type of adhesive or type of slide you use, please take a look at your protocol especially if the sections fall off during HIER and not during routine staining.
René J.  


On Friday, July 18, 2014 10:44 AM, "Murphy, Valerie" <murphyv <@t> karmanos.org> wrote:
  


We are staining human FFPE brain sections for Her 2 Neu and we're having a problem with tissue coming off the slide during the HIER step.
We are currently using Vectabond coated plus slides and using a decloaking chamber for HIER.
Are there any other steps we can take to prevent  tissue coming off the slide?

Thank you,

Valerie Ratliff
Karmanos Cancer Institute


-----------
Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet