[Histonet] Brain tissue coming off slide

Murphy, Valerie murphyv <@t> karmanos.org
Fri Jul 18 09:44:13 CDT 2014

We are staining human FFPE brain sections for Her 2 Neu and we're having a problem with tissue coming off the slide during the HIER step.
We are currently using Vectabond coated plus slides and using a decloaking chamber for HIER.
Are there any other steps we can take to prevent  tissue coming off the slide?

Thank you,

Valerie Ratliff
Karmanos Cancer Institute

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