[Histonet] RE: Storage of HER2 neu control slides

[Joelle Weaver]

Just started using it ( currently doing validation & correlation), so maybe will be issues at some later time. I just noticed the "overnight" in the IFU file from their website when I was putting the SOP together. I have to be honest, that I did my standard preparation so far and it worked great, and had no issues with fall off or staining.  Since it is IVD, was considering in my mind, if shorter baking would be a "modification"? The kit I have uses CB11.  


Joelle Weaver MAOM, HTL (ASCP) QIHC

        
  

 
From: LSebree <@t> uwhealth.org
To: joelleweaver <@t> hotmail.com
CC: histonet <@t> lists.utsouthwestern.edu; Richard.Cartun <@t> hhchealth.org
Subject: RE: [Histonet] RE: Storage of HER2 neu control slides
Date: Mon, 14 Jul 2014 14:59:41 +0000









Thanks Joelle.
 
I’m surprised that Oracle is suggesting overnight baking as I noticed a huge difference when I dried some test tissue controls for 30” @ 60 degrees vs 10” @
 60 degrees.  I was testing some blocks that I had 3 cores  of varying degrees of intensity.  All showed much decreased reactivity at 30” vs 10”.  In fact, the 2 weaker cores were virtually negative.  I can only assume you’re using a different clone than we
 are and that there is different epitope stability between clones.
 
Thanks for your response,
 
Linda
 
 

Linda A. Sebree

University of Wisconsin Hospital & Clinics


IHC/ISH Laboratory


600 Highland Ave.


Madison, WI 53792

(608)265-6596


FAX: (608)262-7174


 


From: Joelle Weaver [mailto:joelleweaver <@t> hotmail.com]


Sent: Monday, July 14, 2014 9:25 AM

To: Cartun, Richard; Sebree Linda A; histonet <@t> lists.utsouthwestern.edu

Subject: RE: [Histonet] RE: Storage of HER2 neu control slides


 

I do the complete drain & dry, date, then refrigeration. Use them within 6 weeks. The Oracle IVD, IFU says bake overnight, but I don't see that happening too often. 




Joelle Weaver MAOM, HTL (ASCP) QIHC



        

  



 

> From: 
Richard.Cartun <@t> hhchealth.org

> To: LSebree <@t> uwhealth.org; 
Histonet <@t> lists.utsouthwestern.edu

> Date: Mon, 14 Jul 2014 14:22:43 +0000

> CC: 

> Subject: [Histonet] RE: Storage of HER2 neu control slides

> 

> We keep all of our positive control slides at RT. The breast predictive marker slides often sit for a few weeks (after cutting) before being used. I think everyone's situation will be different due to laboratory humidity, tissue fixation, and tissue processing
 which removes the water from the tissue which has been shown to be the one of the culprits in antigen degradation in stored unstained slides.

> 

> Richard

> 

> Richard W. Cartun, MS, PhD

> Director, Histology & Immunopathology

> Director, Biospecimen Collection Programs

> Assistant Director, Anatomic Pathology

> Hartford Hospital

> 80 Seymour Street

> Hartford, CT 06102

> (860) 972-1596 Office

> (860) 545-2204 Fax

> richard.cartun <@t> hhchealth.org

> 

> 

> -----Original Message-----

> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree Linda A

> Sent: Monday, July 14, 2014 8:44 AM

> To: Histonet (Histonet <@t> lists.utsouthwestern.edu)

> Subject: [Histonet] Storage of HER2 neu control slides

> 

> Good morning,

> 

> I'd like to get some opinions on the storage of positive control slides for HER2 neu. We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind. What
 has peoples' experiences been with the storage of these control slides?

> 

> Thanks for any and all replies,

> 

> Linda

> 

> 

> Linda A. Sebree

> University of Wisconsin Hospital & Clinics IHC/ISH Laboratory

> 600 Highland Ave.

> Madison, WI 53792

> (608)265-6596

> FAX: (608)262-7174

> 

> 

> 

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