[Histonet] Re: Ventana Ultraview users

[Beth Cox]

Beth,
We are doing exactly that same thing right now. This is changing a 
complete detection system, so we need a full validation (10 pos + 10 
negs for all non-prognostic markers).  To avoid running 20 slides on 
every antibody on our menu ($$$$), we have chosen to use a 3-prong method:

1.  we bought appropriate TMAs from reputable vendors (such as 
Pantomics, Inc)  which will provide more than 10 Pos +10 neg for most cases.
2.  we made a universal "negative" control with 12 different tissues 
from our collection (provides tissues processed by us)
3.  we run 1(or 2) of our control slides (bonus: also validates the 
control at the same time)

This way we only run three slides for each validation to get the 
required number of tissues - big difference in workload and cost!

Beth Cox, HTL/SCT(ASCP)QIHC
Pathology Solutions, Inc
__________________________________________________________________________

Message: 1
Date: Wed, 9 Jul 2014 12:16:55 -0500
From: Beth Brinegar<bbrinegarhtl <@t> gmail.com>
Subject: [Histonet] Ventana ultraview users
To:"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
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Hello all,

Our lab is getting ready to switch from using the iview DAB to the
ultraview - any tips for validation?  How many cases did you run for each
antibody?

Thanks!
Beth Brinegar HTL(ASCP)
Anatomic Pathology Supervisor
Mercy Medical Center
Cedar Rapids, IA 52403