[Histonet] marking tiny specimens

[Barbara Tibbs]

Are you mixing Eosin in the last two alcohols of your processor?   That should work.  If you are dotting the specimens with Eosin before placing in a cassette, the dye will wash out early in the processing.

Also, I don't know if this helps you but when I had to ink mouse arteries I would glue one of my eyelashes to the end of a wood applicator stick and use the eyelash as a tiny paint brush dipped in India ink.  I would do this under a dissecting microscope.  Maybe you could do that with the eggs?

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.tibbs <@t> accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508


________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu <histonet-bounces <@t> lists.utsouthwestern.edu> on behalf of Cheryl Crowder <ccrowder <@t> vetmed.lsu.edu>
Sent: Thursday, January 30, 2014 5:59 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] marking tiny specimens

I am processing some extremely small specimens - pin tip size.  These are
eggs with a protein covering.  I have tried using eosin to color the tissues
before processing but the color came out before paraffin.  The coating on
the eggs will not absorb the dye.  Does anyone have a suggestion for dyeing
or marking these tissues so I can see them better to embed.  Thanks in
advance,
Cheryl

Cheryl Crowder, BA, HTL(ASCP)

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