[Histonet] RE: artifact caused by freezing of tissues during transport

[Tony Henwood (SCHN)]

Hi Carol,
Freezing of tissue whilst in fixative is bad news.
The artefacts can be horrendous. eg:

Rosen, Y., & Ahuja, S. C. (1977). Ice crystal distortion of formalin-fixed tissues following freezing. The American Journal of Surgical Pathology, 1(2), 179-182.

Westenend, P. J. (2004). Incidental freezing artefacts in sentinel lymph node biopsies masquerading as lymphangiography artefacts. Journal of clinical pathology, 57(6), 671.



Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 

Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol Bryant
Sent: Tuesday, 28 January 2014 3:44 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] artifact caused by freezing of tissues during transport

Has anyone experienced artifact caused by freezing of tissues during transport with these frigid days?  If so, how long does it take specimens submitted in 10% NBF to freeze while being transported to a laboratory?





Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com



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