[Histonet] zebrafish embryos histology

[Jack Ratliff]

Patty,
I have personally never performed histology on zebra fish embryo's, but if I was to test it out on my own I might try using the JB4 Plus GMA kit. It just seems to me that paraffin might cause too much shrinkage and maybe using an MMA protocol might be too harsh on the tissue. Besides, given the hydrophilic nature of GMA it just might be the best overall solution.
Best,
Jack


> From: plott <@t> uab.edu
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Wed, 22 Jan 2014 15:00:40 +0000
> Subject: [Histonet] zebrafish embryos histology
> 
> Can anyone give me a method for zebra-fish embryo histology?  The papers I've read show photos, but no description of histology in M & M.  I need to put several embryos in each block, and get the orientation correct, and put multiple sections on each slide, in hopes of getting one or two that are perfect.  Any suggestions would be greatly appreciated.
> Thanks,
> Patty Lott
> UAB CMBD Core Lab
> 205-934-2007
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