[Histonet] Cell Marque Mammoglobin (31A5) New Protocol?

[joelle weaver]

also have IVD mammoglobin, clone  31A5 rabbit monoclonal, works really well for me at 1:75-  if you need/prefer IVD from cancer diagnostics, the other I posted is ASR. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: mrs.abby.maples <@t> gmail.com
> Date: Wed, 22 Jan 2014 08:11:11 -0600
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol?
> 
> Fellow histotechs,
> 
> We've been running mammoglobin testing on our Ventana Benchmark XT.
> However, Cell Marque (our supplier of mammoglobin antibody) has changed the
> concentration. Our old concentration was 0.12 mg/mL, the new concentration
> is weaker at 0.04 mg/mL. The antibody is a rabbit monoclonal. We tried
> running the old protocol with the new concentration, but the results were
> not adequate (see below). We tried running the protocol given to us by Cell
> Marque, but theirs is specific towards the Benchmark UltraView and also
> returned inadequate results.
> 
> We process our tissue from formalin to paraffin. Our lab uses the iVew DAB
> detection kit.
> 
> Our old protocol:
> Deparaffinization
> Conditioner #1 (short 8 minute conditioning)
> Mild CC1 (mild 30 minutes conditioning)
> Standard CC1 (standard 60 minute conditioning)
> No enzyme
> 37C antibody incubation temperature
> No titration
> Antibody: Mammoglobin 31A5 at 0.12 mg/mL concentration for 32 minutes
> A/B Block
> Hematoxylin counterstain 4 minutes
> Blueing post counterstain 4 minutes
> 
> Does anyone else use this product from Ventana/Cell Marque? Does anyone
> have a working protocol for a weaker antibody titration?
> 
> Any help would be greatly appreciated!
> 
> *Abby L. H. Maples, BS, MPH, HTL (ASCP)CM*
> Histotechnologist, Surgical Pathology
> Mercy Medical Center
> 701 10th Street SE
> Cedar Rapids, IA 52403
> Lab Phone: (319) 398-6827
> Cell: (319) 432-1530
> Fax: (319) 221-8767
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