[Histonet] RE: TAT for Transplant Biopsies

[Tony Henwood (SCHN)]

Hi Richard,

Unfortunately we do not have a cut-off time for urgent transplant biopsies (its seems a kid's hospital is different).
We microwave process kidney, lung and liver biopsies, about 30 minutes in total, though if we can get away with it we extend this by 30 minutes for more fixation, using 10%NBF and microwave.
We use a Micromed TT.

We can then do this out of hours and weekends - cost a bit in overtime though, but hopefully it is worth it. 
The morphology is nearly always better than routine processing (using ethanol and xylene) and my pathologists prefer this for routine renal biopsies as well. There seems to be less leaching out of cytoplasmic substances compared to the routinely processed biopsies.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 

Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Friday, 10 January 2014 11:47 AM
To: Histonet
Subject: [Histonet] TAT for Transplant Biopsies

For those of you seeing biopsies of transplant kidney, liver, and heart, what is your cut-off time for accepting a specimen for same-day processing?  Do any of you handle specimens at night, weekends, or holidays?  Thank you!



Richard



Richard W. Cartun, MS, PhD

Director, Histology & Immunopathology

Director, Biospecimen Collection Programs

Assistant Director, Anatomic Pathology

Hartford Hospital

80 Seymour Street

Hartford, CT  06102

(860) 972-1596 Office

(860) 545-2204 Fax

richard.cartun <@t> hhchealth.org<mailto:richard.cartun <@t> hhchealth.org>

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