[Histonet] Betagalactosidase/LacZ

[Elizabeth Cameron]

I have a question Lac Z staining and BetaGalactosidase IHC.  The Lac-Z stain is enzyme dependent, so it  must be fresh/frozen for the reaction to work.  Is this also true for the betagalactosidase IHC?  The target of the antibody is the enzyme, but I am not sure if the enzyme need to be "active" or just present for the antibody to bind to it.  If anyone has knowledge on the subject, please share!  We are working with mouse kidneys, and although our controls are positive, the kidneys that should be are not.
Thank you,
Liz

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