[Histonet] question about refrigeration

[Sean McBride]

Hi Peggy,

In my opinion, it depends upon the objective of the study. Cell autolysis begins shortly after death as a result of lack of oxygen supply as well as nutrients.  The membranes of the lysosomes break down, and the acid hydrolases begin to degrade the cellular 

If you're merely looking to do a macro analysis, such as the quantitation of bone, you should be fine. However, if you're hoping to get results at the cellular level, either with traditional stains or IHC which relies on intact protein structure, you should immerse the specimens  immediately after retrieval into formaldehyde solution. Good luck.

Best regards,

Sean

Sean McBride
AFIRM Project Leader
Senior Scientific Specialist
Research Histology Services
Bone Tissue Engineering Center
Carnegie Mellon Research Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124

571-989-BONE (m)
412-268-8275 (o)
412-268-8275 (fax)
smcbride <@t> andrew.cmu.edu 







On Feb 28, 2014, at 9:12 AM, DiCarlo, Margaret wrote:

> Histonetters,
> 
> I was wondering how long a bone specimen (portion of pt's tibia in this case) can be in a refrigerator before it will decompose and not produce a good quality section?  The case was completed at 3:30 pm, refrigerated and then put into formalin at 8:15 am the next day?  I am pushing to put specimen into formalin immediately after surgery.
> 
> Thank you.
> 
> Peggy DiCarlo HT (ASCP)
> Ortho Bone Lab
> Buffalo General Hospital
> Buffalo, NY  14203
> 716-859-1293
> 
> 
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