[Histonet] microscope information

[James Watson]

If you drop the condenser down so Koehler illumination is not aligned, this give you a pseudo phase contrast.   You can see many structures in tissue due to the different density of the cells.  So I would recommend a microscope that has a condenser that can be moved up and down.  We have 2 old Olympus BH2 in our Microtomy area.

James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation
Tel    858-332-4647
Fax   858-812-1915
jwatson <@t> gnf.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, February 27, 2014 9:57 AM
To: Histonet <@t> lists.utsouthwestern.edu; Lisa*Freeman
Subject: Re: [Histonet] microscope information

ANY microscope with decent objectives will do. Even used microscopes you can buy in eBay.
The problem is not with the microscope but how the HTs will be able to locate those structures while still embedded in the paraffin.
René J.


--------------------------------------------
On Thu, 2/27/14, Freeman, Lisa* <Lisa.Freeman <@t> fda.hhs.gov> wrote:

 Subject: [Histonet] microscope information
 To: "Histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
 Date: Thursday, February 27, 2014, 12:46 PM
 
 I am currently looking for a
 microscope to use at the sectioning stations. We have to  locate tissues such as parathyroid glands and mammary glands  at sectioning, as well as general QC. Any opinions on which  is the best for this purpose?
 
 Thank you,
 
 Lisa Freeman, HT
 Histology Supervisor
 Toxicologic Pathology Associates
 National Center for Toxicological Research
 3900 NCTR RD
 Jefferson, AR 72079
 Phone: 870-543-7234
 E-mail: Lisa.Freeman <@t> fda.hhs.gov
 
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