[Histonet] aqueous mounting medium

[Mehmet Fatih BOZKURT]

I'm using modified protocol. I'm storing it at 4C.


*Aqueous Mounting Medium (glycerol gelatin)*


 *Kisser's**Kaiser's**Glycerol jelly**Modified*Gelatine10 g40 g65 g5 gDistilled
water35 ml210 ml300 ml50 mlGlycerol (glycerin)30 ml250 ml100 ml50 mlPhenol
(carbolic acid) 5 ml5 ml





Mix gelatin and distilled water. Heat in 50 C oven until gelatin is
dissolved (it takes about 30 minutes). Add glycerin and adjust pH to 7.0
using 1N NaOH (to avoid fading of hematoxylin). Store at room temperature.



Warm to 37-40 C before mounting. Note: Modified protocol works well.



from ihcworld.com





On Wed, Feb 19, 2014 at 8:22 PM, Caroline Miller <cmiller <@t> gladstone.ucsf.edu
> wrote:

> I use prolong gold from life tech (formerly molecular probes and now part
> of the world dominating thermofisher) without Dapi as I do that separately,
> for IF and aquaslip from American master tech for non IF aqueous mounting
>
> Good luck!
> Caroline
>
> Caroline Miller
> Gladstone Institutes
> www.gladstoneinstitutes.org
>
> Tel: 415 7342566
> Cell: 415 2187297
>
>
>
>
> > On Feb 19, 2014, at 10:11 AM, Hans B Snyder <hans <@t> histologistics.com>
> wrote:
> >
> > Hello,
> >
> > I have one for ORO but not for IF.  If someone has one for IF, I would
> be interested also.
> >
> > Thank you
> >
> > Histologistics
> > Hans B Snyder
> > 508.308.7800
> > Hans <@t> histologistics.com
> >
> >> On Feb 19, 2014, at 12:27, Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
> wrote:
> >>
> >> Does anyone have a good "recipe" for aqueous mounting medium?
> >> Thanks,
> >> Jennifer
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-- 
Asst. Prof. Dr. Mehmet Fatih BOZKURT
Department of Pathology
Faculty of Veterinary Medicine
Afyon Kocatepe University
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-16173/16237

Asst. Prof. Dr. Mehmet Fatih BOZKURT
Manager of Veterinary Diagnostic Laboratory
Afyon Kocatepe University
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-16300-16301
vetanaliz <@t> aku.edu.tr