[Histonet] BBB permeability marker
Hilary Smith
hsmith <@t> wakehealth.edu
Tue Feb 18 10:18:01 CST 2014
Hello - does anyone have a recommendation for a marker of blood brain barrier disruption in fresh frozen cryostat sectioned tissue? Can I postfix and immunostain for albumin extravasation, or does albumin easily diffuse away from the vessels post-mortem? The tissue was flash frozen in isopentane, however it was thaw-mounted onto slides before overnight dessication and refreezing.
Thanks,
Hilary
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