[Histonet] Re: Histonet Digest, Vol 123, Issue 15

Karen k.leigh.adams.865 <@t> gmail.com
Sun Feb 16 12:36:25 CST 2014


Yves,

    You did not mention the melting point of your paraffin...any temperature above 60 degrees or prolonged exposure of heat may destroy protein in the tissue causing structurally damaged antigenic sites. This would invalidate your IHC results as staining would not be representative of what was originally present.

Leigh

Sent from k. Leigh's iPad.

> On Feb 15, 2014, at 1:00 PM, histonet-request <@t> lists.utsouthwestern.edu wrote:
> 
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> Today's Topics:
> 
>   1. RE: TJC on IHC Neg Controls (Terri  Braud)
>   2. HISTOPALOOZA  April 25 - 27, 2014 (Zimmerman, Billie)
>   3. In need of Amyloid Control Tissue (Nani Brabson)
>   4. End time for fixation of breast tissue (Glenn Hauck)
>   5. RE: End time for fixation of breast tissue (Morken, Timothy)
>   6. ANP. 23045 performance of all instuments (anita)
>   7. adverse effect of prolonged paraffin embedding on    staining ?
>      (Yves Heremans)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Fri, 14 Feb 2014 13:31:01 -0500
> From: "Terri  Braud" <tbraud <@t> holyredeemer.com>
> Subject: [Histonet] RE: TJC on IHC Neg Controls
> To: <histonet <@t> lists.utsouthwestern.edu>
> Cc: Erin.Martin <@t> ucsf.edu
> Message-ID:
>    <BFBF7C6B9627B947B74CBEBD45CE368B1246C1D3 <@t> hrex-svr.holyredeemer.local>
> Content-Type: text/plain;    charset="us-ascii"
> 
> I don't know who your manager talked to, but he/she has been
> misinformed.  There is no such specific Joint Commission Standard. Here
> is what they state, copied from their standards:
> QCP.2.1.1 When immunohistochemistry is performed, the laboratory has
> appropriate
> quality control processes.
> Also, TJC defers to CAP regulations
> 
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Holy Redeemer Hospital Laboratory
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> Ph: 215-938-3676
> Fax: 215-938-3874
> 
> 
> Message: 4
> Date: Thu, 13 Feb 2014 18:20:59 +0000
> From: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
> Subject: [Histonet] Negative Controls
> 
> 
> Hi All,
> We are a Joint Commission inspected lab but after CAP changed their
> requirements for negative controls we were hoping to drop them too. My
> manager contacted TJC and asked what their position was regarding
> negative controls.  They responded that they have not changed their
> requirements and therefore still  want to see negative controls.  Has
> anyone by inspected recently by TJC and had this issue come up?
> Thank you!
> Erin Martin
> Erin Martin, Histology Supervisor
> UCSF  Dermatopathology Service
> 415-353-7248
> ---------------------------------------------------------------------------------
> 
> 
> 
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> 
> ------------------------------
> 
> Message: 2
> Date: Fri, 14 Feb 2014 18:31:59 +0000
> From: "Zimmerman, Billie" <BZIMMERM <@t> gru.edu>
> Subject: [Histonet] HISTOPALOOZA  April 25 - 27, 2014
> To: "histonet <@t> lists.utsouthwestern.edu"
>    <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>    <7B3DEB32E69C034EACB479059C5DE3FF810636 <@t> EX-MLB-03.ad.georgiahealth.edu>
>    
> Content-Type: text/plain; charset="us-ascii"
> 
> I hope all of you wondered where I've been. I have survived the winter ice storm in Augusta, Georgia.  Let's just say if Jim Cantore, from the Weather Channel, arrives in your city, you know it's the kiss of death!   When I heard he had  arrived, I knew we were doomed.
> But, I'm baaaack! Today's feature is a tag team, Jim and Theresa Burchette. Jim will be conducting a workshop titled "Immunohistochemical Detection of Infectious Diseases."  The brochure  says audience participation is encouraged.  I have no idea what that could mean.  It's either sharing of proper staining patterns  or how to hand  tie a tootie fruitie fly for fishing. Theresa's presentation is titled "Immunoreactivity in Normal Tissue".  This is an extremely helpful workshop for selecting and maintaining IHC tissue controls.
> Both of these workshops will serve as great contact hours if you are wanting to maintain your QIHC or are interested in learning about IHC.  Jim and Theresa are both wonderful speakers and I promise you won't have to play candy crush under the table to stay awake.
> 
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Fri, 14 Feb 2014 14:21:33 -0500
> From: Nani Brabson <nbrabson <@t> MPLNet.com>
> Subject: [Histonet] In need of Amyloid Control Tissue
> To: "'histonet <@t> lists.utsouthwestern.edu'"
>    <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>    <908827E7E3D5094BA8495A3D40ADEFE00137DC387E7C <@t> MAIL01.mplnet.com>
> Content-Type: text/plain; charset="us-ascii"
> 
> Does anyone have any Amyloid tissue they are willing to share?  We are a small lab and Amyloid is very hard for us to come by.
> Thanks,
> Nani
> Maryville, TN
> 
> 
> 
> 
> ------------------------------
> 
> Message: 4
> Date: Fri, 14 Feb 2014 13:18:18 -0700
> From: Glenn Hauck <Glenn.Hauck <@t> albertahealthservices.ca>
> Subject: [Histonet] End time for fixation of breast tissue
> To: "'histonet <@t> lists.utsouthwestern.edu'"
>    <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>    <38FA8FCA474F834CB9B90590D7C72390015A681C5C4B <@t> EXMBX4C.crha.bewell.ca>
> Content-Type: text/plain; charset="us-ascii"
> 
> Is there someone out there that can help me find out if there is any value in recording the end time for fixation of breast tissue in 10% formalin?
> 
> We at present record the devitalization time, the time tissue is put into fixative and the time the tissue is cut into and exposed to 10% formalin as well as the time the processor is started
> 
> Thanks
> 
> 
> Glenn Hauck
> Charge Tecnologist
> Pathology
> QueenElizabeth II Hospital
> Grande Prairie, AB T8V 2E8
> 
> 780-538-7429 Work Main
> 780-538-7184 Work Office
> glenn.hauck <@t> albertahealthservices.ca
> 
> 
> 
> ________________________________
> This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you.
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Fri, 14 Feb 2014 20:40:14 +0000
> From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
> Subject: [Histonet] RE: End time for fixation of breast tissue
> To: "'Glenn Hauck'" <Glenn.Hauck <@t> albertahealthservices.ca>
> Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>    <761E2B5697F795489C8710BCC72141FF36758028 <@t> ex07.net.ucsf.edu>
> Content-Type: text/plain; charset=us-ascii
> 
> Glenn, You do need to show it was in formalin for at least 6 hours. we work backwards from the time on the processor that the tissue leaves the formalin. For us 3pm is the cutoff for breast tissue to get 6 hours fixation.
> 
> Tim Morken
> Supervisor, Electron Microscopy and Neuromuscular Special Studies
> UC San Francisco Medical Center
> San Francisco, CA
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Glenn Hauck
> Sent: Friday, February 14, 2014 12:18 PM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] End time for fixation of breast tissue
> 
> Is there someone out there that can help me find out if there is any value in recording the end time for fixation of breast tissue in 10% formalin?
> 
> We at present record the devitalization time, the time tissue is put into fixative and the time the tissue is cut into and exposed to 10% formalin as well as the time the processor is started
> 
> Thanks
> 
> 
> Glenn Hauck
> Charge Tecnologist
> Pathology
> QueenElizabeth II Hospital
> Grande Prairie, AB T8V 2E8
> 
> 780-538-7429 Work Main
> 780-538-7184 Work Office
> glenn.hauck <@t> albertahealthservices.ca
> 
> 
> 
> ________________________________
> This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you.
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> 
> 
> ------------------------------
> 
> Message: 6
> Date: Fri, 14 Feb 2014 16:33:22 -0600
> From: anita <azdudley <@t> hotmail.com>
> Subject: [Histonet] ANP. 23045 performance of all instuments
> To: "Histonet <@t> lists.utsouthwestern.edu"
>    <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <COL131-W55909D4F0A6E3CABE12061D19C0 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
> 
> What are others saying in your procedures for this?  Not sure just to put they should all be checked to see if running the way they should be?  Thanks for the help, everyone have a great weekend!!!
> 
> 
> 
> Anita Dudley
> 
> Mobile, AL.
>                         
> 
> ------------------------------
> 
> Message: 7
> Date: Sat, 15 Feb 2014 16:26:49 +0100
> From: Yves Heremans <Yves.Heremans <@t> vub.ac.be>
> Subject: [Histonet] adverse effect of prolonged paraffin embedding on
>    staining ?
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <28E38E81-985F-4357-AE65-F0E0369F73F1 <@t> vub.ac.be>
> Content-Type: text/plain; charset=us-ascii
> 
> Dear all,
> 
> Is there any (published ?) negative effect when tissues stay in hot paraffin over the weekend on antibody staining (diminished staining intenstity ?) ?
> 
> Yves
> 
> 
> ------------------------------
> 
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> End of Histonet Digest, Vol 123, Issue 15
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