[Histonet] RE: Mouse F4/80 antibody

[koellingr <@t> comcast.net]

Hello, I agree anti-human CD68 might not be best for mouse tissue but there are specific mouse CD68 (the mouse homolog to human CD68) antibodies out there.  FA-11 is one of my favorites.  Have used both F4/80 and (rat anti-mouse CD68) on flow, frozens and FFPE.  Anna, a literature search gives you published  protocols.  I'm looking right now at a photo of some CD68+ macs staining on mouse tissues I took from years back.  Shows them nicely.  anti-mouse CD68 on mouse tissue is in the literature. 
  
Ray 
Seattle 

----- Original Message -----

From: pruegg <@t> ihctech.net 
To: "Anna Hughes" <anna.c.hughes <@t> gsk.com>, histonet <@t> lists.utsouthwestern.edu 
Sent: Wednesday, February 12, 2014 12:02:07 PM 
Subject: [Histonet] RE: Mouse F4/80 antibody 

Rat anti ms F4/80 is used on mouse tissue ffpe for macrophages not anti 
human cd68. 

Patsy Ruegg, HT(ASCP)QIHC 
Ruegg IHC Consulting 
40864 E. Arkansas Ave 
Bennett, CO 80102 
H 303-644-4538 
C 720-281-5406 
pruegghm <@t> hotmail.com 

-----Original Message----- 
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anna Hughes 
Sent: Wednesday, February 12, 2014 9:14 AM 
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] Mouse F4/80 antibody 

Hi Everyone! 
I was wondering if any of you have a favorite CD68 macrophage antibody that 
you have used on mouse tissue and is especially reliable.  This is a target 
that has been notorious in our lab and I was wondering if there was a really 
good one to use. 

Thanks in advance for your help! 

Anna Hughes 
Anna C. Hughes, BBA, BS, HTLCM 
anna.c.hughes <@t> gsk.com 


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