[Histonet] RE: Leica Cryostat CM 1860 UV Decontamination Procedure

[Keyser Gerald T]

We use a Leica CM1850 currently. May differer a bit. On a daily basis, we clean the loose trimmings and wipe the inside with 95% ethanol. The exterior surfaces are cleaned daily with caviwipes. 

Once a month we turn off the machine, remove the microtome (I don't know if this is proper for the CM1860), let it completely defrost. Then clean the entire chamber with 95% ethanol. The microtome is dyed completely and the cryostat reassembled. 

This procedure was in place long before I started working where I do. I'm not sure if this procedure is something that is leica approved. I believe the CM1860 has a different microtome construction than the CM1850. 

I'm also hoping that the next cryostats we get have UVC. I really like the idea of UVC. 


________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Adesupo, Adesuyi (Banjo) [abadesuyi <@t> nrh-ok.com]
Sent: Wednesday, February 12, 2014 11:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Leica Cryostat CM 1860 UV Decontamination Procedure

Hi,
    How are you guys doing? Please I am wondering if some of you guys are using this type of Leica cryostat and would be interested in the decontamination procedure that you have in place.

 Thanks,

  Adesupo Adesuyi, BSMT, HT (ASCP), HTL (ASCP), QIHC (ASCP)
  Histology Supervisor
  Norman Regional Health System,
  Norman, OK 73071.
  Tel: 405- 307- 1145


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