[EXTERNAL] RE: [Histonet] Purple haze on H&E slides

[McAnn, Sherrian]

We once had this problem..We routinely filter our hematoxylin every
morning at startup.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Burrage,
Shannon L.
Sent: Tuesday, February 11, 2014 9:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [EXTERNAL] RE: [Histonet] Purple haze on H&E slides

Are you using acid alcohol, and Harris Hematoxylin? Are you filtering
the heamatatoxylin every day? We still use a very small amount of
gelatin in the waerbath. And get no background stain. So many veriables
here.
Good luck,
Shannon Burrage
shannon.burrage <@t> unitypoint.org

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Campbell, Tasha
M. [tmcampbell <@t> fmh.org]
Sent: Monday, February 10, 2014 7:37 PM
To: Huggins, Haley - MRMC
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Purple haze on H&E slides

What brand of reagents are u using and what is your protocol? What
slides r u using and r u using gelatin in ur waterbath?

Tasha

Sent from my iPhone

> On Feb 10, 2014, at 7:10 PM, "Huggins, Haley - MRMC"
<Haley.Huggins <@t> DignityHealth.org> wrote:
>
> I am trying to get the histology lab up and running and I am having
difficulties getting the H&E Stain just right. I am nearly there, but I
keep getting a purple bleed or haze around the tissue. Any suggestions
on getting rid of this? I actually got rid of it at one point, but the
medical director wasn't happy with the stain. I appreciate any
suggestions from the histology world.
>
> Haley Huggins
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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