[Histonet] Buffer pH monitoring

[Gupta, Nilesh]

I just want to get an idea of how often labs monitor the pH of the buffers used in IHC labs. Do you pH for every bottleof a lot received prior to use, single bottle of a lot only or do you have to pH every time you reconstitute the buffer.

It is a CAP checklist question for AP & here is how the checklist question is worded. It says when a new "batch" is prepared or received. Any input is greatly appreciated. Thanks Nilesh Gupta, M.D, Henry Ford Hospital, Detroit, MI

The pH of the buffers used in immunohistochemistry is routinely monitored.

NOTE:  pH must be tested when a new batch is prepared or received.

Evidence of Compliance:

✓      Written procedure defining pH range for each buffer in use AND

✓      Records of initial and subsequent QC on each buffer



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