[Histonet] RE: Fungus contamination

Tue Feb 4 12:49:22 CST 2014

I have had fungal contamination in the Fast Green counterstain from Thermo.

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N
Sent: Tuesday, February 04, 2014 10:33 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: Fungus contamination

Judith,
What counterstain are you using for each?  If it is the light green, then that may be the culprit.  Also check your baskets that you to deparaffinze, they may need to be cleaned.

Sincerely,

Toysha N. Mayer, MBA, HT(ASCP)
tnmayer <@t> mdanderson.org
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
713-563.3481

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Message: 8
Date: Tue, 4 Feb 2014 15:55:11 +0000
From: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>
Subject: [Histonet] Fungus contamination
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
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	<F8593D93099A864A9730359FA6992A87475B64B8 <@t> CHIEX016.CHI.catholichealth.net>

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Having a problem with fungus on top of both the pas and gms stain. There are no solutions used on both test. Other than distilled water we can not figure it out. All solutions have been changed and clean containers used.

Judith Gale Pardue
Histology Supervisor
judith_pardue <@t> memorial .org

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Message: 9
Date: Tue, 4 Feb 2014 09:11:35 -0700
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: [Histonet] RE: Fungus contamination
To: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
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	<14E2C6176416974295479C64A11CB9AE019C79E0529B <@t> SBS2K8.premierlab.local>
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Judith

Its been years ago but I wrote a ASCP tech sample on this - I can't remember what solution it was but one of the solutions we used for the GMS stain actually grew fungus in it and we were getting staining on top of the tissue sections also.  If you don't think it's one of your solutions I would culture your distilled water.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com

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Message: 10
Date: Tue, 4 Feb 2014 16:21:32 +0000
From: "Truscott, Tom" <ttruscot <@t> vetmed.wsu.edu>
Subject: [Histonet] RE: Fungus contamination
To: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<9EF5279EBDFE6E4FB6605E8F183A00276C8F61EC <@t> CVM77.vetmed.wsu.edu>
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Hi Judith, If you have made completely fresh solutions, even any stock reagents and still have the problem, then the fungus may be arriving from another source. I once had a problem of pollen landing on my water bath and showing up on the stains that would detect it. You may have fungus on other slides but only showing up in the PAS and GMS. Tom T

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pardue, Judith
Sent: Tuesday, February 04, 2014 7:55 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fungus contamination

Having a problem with fungus on top of both the pas and gms stain. There are no solutions used on both test. Other than distilled water we can not figure it out. All solutions have been changed and clean containers used.

Judith Gale Pardue
Histology Supervisor
judith_pardue <@t> memorial .org

This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.
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