[Histonet] Antibody PD-1 - IHC

avistarop <@t> ffyb.uba.ar avistarop <@t> ffyb.uba.ar
Tue Feb 4 09:23:04 CST 2014


Hello to everyone!

Has anyone used PD-1 on FFPE tissue?  Could I please get a copy of your
IHC protocol?

 I would like to know:

* antigen retrieval: method, retrieval buffer and times
* primary antibody incubation: antibody diluyent (did you use detergent?
tween or other?) and incubation time.

Thank you so much.

Aldana Vistarop






More information about the Histonet mailing list