[Histonet] Warthin Starry on Dako Artisan

[Cooper, Brian]

Hi, 

We've had really good results with pretreatment in 1% Uranyl Nitrate, as well as with 1% Uranyl Acetate in Acetate Buffer--both at room temperature, and at 60 degrees C for 10 minutes.  We've also tried zinc formalin, as well as "Spirochete Sensitizer"--a commercially available product, but we've only obtained extremely faint staining of spirochetes with the latter two.   Our sections are cut at 4 µm.   
 
Thanks, 

Brian

-----Original Message-----
From: Sanders, Jeanine (CDC/OID/NCEZID) [mailto:jqb7 <@t> cdc.gov] 
Sent: Wednesday, December 24, 2014 5:13 AM
To: Cooper, Brian; 'histonet <@t> lists.utsouthwestern.edu'
Subject: RE: [Histonet] Warthin Starry on Dako Artisan

What protocol with uranyl nitrate do you use? Dako told us to use zinc formalin as a sensitizer but it doesn't seem to make much of a difference. Thanks!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, December 23, 2014 11:18 AM
To: Cooper, Brian; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Warthin Starry on Dako Artisan

Try a 0.10% solution of phosphotungstic acid.René J.  

     On Monday, December 22, 2014 6:39 PM, "Cooper, Brian" <bcooper <@t> chla.usc.edu> wrote:
   

 Happy Holidays Histonetters!

Has anyone out there successfully stained spirochetes with Dako's Artisan Warthin-Starry kit, without first pretreating the sections with uranyl nitrate?  With offline uranyl nitrate pretreatment, spirochetes stain brilliantly!  But everything else we've tried leaves them invisible . . .

Any guidance would be greatly appreciated.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357    Pager: 213-209-0184
bcooper <@t> chla.usc.edu<mailto:bcooper <@t> chla.usc.edu>



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