[Histonet] Warthin Starry on Dako Artisan

[Rene J Buesa]

Try a 0.10% solution of phosphotungstic acid.René J.  

     On Monday, December 22, 2014 6:39 PM, "Cooper, Brian" <bcooper <@t> chla.usc.edu> wrote:
   

 Happy Holidays Histonetters!

Has anyone out there successfully stained spirochetes with Dako's Artisan Warthin-Starry kit, without first pretreating the sections with uranyl nitrate?  With offline uranyl nitrate pretreatment, spirochetes stain brilliantly!  But everything else we've tried leaves them invisible . . .

Any guidance would be greatly appreciated.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357    Pager: 213-209-0184
bcooper <@t> chla.usc.edu<mailto:bcooper <@t> chla.usc.edu>



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