[Histonet] Warthin Starry on Dako Artisan

Rene J Buesa rjbuesa <@t> yahoo.com
Tue Dec 23 10:17:34 CST 2014


Try a 0.10% solution of phosphotungstic acid.René J.  

     On Monday, December 22, 2014 6:39 PM, "Cooper, Brian" <bcooper <@t> chla.usc.edu> wrote:
   

 Happy Holidays Histonetters!

Has anyone out there successfully stained spirochetes with Dako's Artisan Warthin-Starry kit, without first pretreating the sections with uranyl nitrate?  With offline uranyl nitrate pretreatment, spirochetes stain brilliantly!  But everything else we've tried leaves them invisible . . .

Any guidance would be greatly appreciated.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357    Pager: 213-209-0184
bcooper <@t> chla.usc.edu<mailto:bcooper <@t> chla.usc.edu>



---------------------------------------------------------------------
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, 
is for the sole use of the intended recipient(s) and may contain confidential
or legally privileged information. Any unauthorized review, use, disclosure
or distribution is prohibited. If you are not the intended recipient, please
contact the sender by reply e-mail and destroy all copies of this original message.  

---------------------------------------------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

   


More information about the Histonet mailing list