[Histonet] Toluidine blue background

[O'neil, Beth]

We would like to use Toluidine Blue for staining frozen sections of kidney biopsy.  We finally are able to get the stain dark enough, but we can't get rid of the background caused by the OCT.    We've washed with water, alcohols, and PBS  with no success.  Is anyone out there using Toluidine Blue for frozen staining?  If so, what is your recipe and how do you get rid of the background?
Thanks

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
oneilb <@t> wvuhealthcare.com<mailto:oneilb <@t> wvuhealthcare.com>
Histology Supervisor, Technical Specialist
Lab:  304 - 293 - 6014
Office:  304 - 293 - 7629



-----------------------------------------
Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information.  Any unauthorized review, use, disclosure or distribution is prohibited.  If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.