[Histonet] Shelf life
raghulj <@t> orchidpharma.com
raghulj <@t> orchidpharma.com
Thu Dec 11 23:22:25 CST 2014
Hi Angela,
We came across similar situation during our GLP inspection. As Morken has mentioned the validation would certainly stand by us, more than the expiry dates in the bottle. We give 3 months expiry for any reagents prepared in our lab and usually it get consumed by that time. I would suggest to fix a purpose for the prepared solution and validate it to the purpose, say by checking the pH when trying to use the HCl solution to differentiate.
Hope this was of some help. All the best
Regards
raghul
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: 10 December 2014 23:37
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Subject: Histonet Digest, Vol 133, Issue 12
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Today's Topics:
1. Manager Opportunity - Birmingham, AL (Casey Sorrell)
2. cutting MMA cross section of mouse femur (Dorothy Hu)
3. Difference Between Histotech Lead and Histology Supervisor
Positions (Joseph Mecham)
4. shelf life (Bitting, Angela K.)
5. RE: shelf life (Morken, Timothy)
6. Solution Chemistry - Alcoholic Formalin - Bone Histology -
Fluorescent Label (Hernandez, Jesus Willibaldo)
7. Re: shelf life (Rene J Buesa)
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Message: 1
Date: Tue, 9 Dec 2014 17:01:40 -0600
From: Casey Sorrell <csorrell <@t> skindxgroup.com>
Subject: [Histonet] Manager Opportunity - Birmingham, AL
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <D0ACDB74.B02%csorrell <@t> skindxgroup.com>
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Skin Diagnostics Group, PC, is currently accepting candidate resumes for a Dermatopathology Laboratory Manager.
POSITION TITLE: Manager of Lab Operations
LOCATION: Birmingham, AL
To apply: Please send resume to amoncrief <@t> skindxgroup.com<mailto:amontcrief <@t> skindxgroup.com>
Job Summary:
Manage 25+ Lab Technicians and Lab Assistants (2 shifts) in a high volume, national skin pathology laboratory. Maintain accreditations with CLIA, College of American Pathologists (CAP). This individual will use their abilities to motivate a staff to insure that high quality, cost effective services are consistently delivered and will be accountable for daily lab operational management, training, goal setting, prioritization, quality & process improvement, instrument evaluation/maintenance, and customer service.
Qualifications:
BS degree preferred, HT (ASCP), 5+ years experience in high volume Anatomic Pathology laboratory, preferably Dermatopathology, with minimum 3 years of successful supervisory/management experience. Excellent computer skills along with strong leadership ability, problem solving and resolution, teamwork, and excellent communication skills.
Salary:
Highly competitive compensation and benefits with relocation assistance.
K. Casey Sorrell
Chief Operating Officer
3512 Old Montgomery Highway
Birmingham, AL 35209
205-879-2260
www.skindxgroup.com<http://www.skindxgroup.com>
------------------------------
Message: 2
Date: Wed, 10 Dec 2014 09:25:17 -0500
From: Dorothy Hu <abtdhu <@t> gmail.com>
Subject: [Histonet] cutting MMA cross section of mouse femur
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Cc: Dorothy Hu <abtdhu <@t> gmail.com>
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Dear Histonetters,
I normally use 85/15 ratio of MMA/dibutyl phthalate (DP) in my mouse tibia and femur longitudinal sections. But for cross section of mouse femur. Do you change the ratio of MMA/DP to make block more soft or hard? It is difficulty to get good cross sections. Any expert especially from hard tissue committee, please help. I'm very much appreciated.
Dorothy Hu
MGH endocrine
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Message: 3
Date: Wed, 10 Dec 2014 15:28:36 +0000
From: Joseph Mecham <dmecham <@t> copc.net>
Subject: [Histonet] Difference Between Histotech Lead and Histology
Supervisor Positions
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
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<E00DB5A2C97EA54E8578D67D73F3FBC115133B0A <@t> COPC-SBS.COPC.local>
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I am looking for some opinions on how everyone's individual labs view the Lead tech and Histology Supervisor positions. Would love everyone's input. I know NSH's salary scale shows a larger variance in pay between the two.
Thanks in advance!
Drew Mecham
Central Oregon Regional Pathology Services
1348 NE Cushing Drive
Bend, OR 97701
541.693.2651
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Message: 4
Date: Wed, 10 Dec 2014 16:45:03 +0000
From: "Bitting, Angela K." <akbitting <@t> geisinger.edu>
Subject: [Histonet] shelf life
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We are being inspected soon and I'm trying to check the labels on all of my chemicals. How do I determine the expiration date of a 1N HCl solution I prepared in house. I went on line and a commercially available 1N HCl solution has a 36 month shelf life according to the vendor's web site. I don't know how they arrived at that. I know concentrated HCl has an indefinite shelf life, but a 5 year retest date appears on the COA. It's confusing to me.
Any help would be appreciated.
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Message: 5
Date: Wed, 10 Dec 2014 17:00:14 +0000
From: "Morken, Timothy" <Timothy.Morken <@t> ucsf.edu>
Subject: [Histonet] RE: shelf life
To: "'Bitting, Angela K.'" <akbitting <@t> geisinger.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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Angela most of these "shelf life" dates are just the length of time a vendor is willing to be responsible for the reagent. It's mostly a financial decision, not chemical deterioration. They just don't want to be responsible for chemicals for too long. So that is why you see variations in shelf life or expiration dates for the same chemical from different vendors.
We generally give any solution we make up a 6-month shelf life unless we know from validations that is it is shorter. We make up solutions in amounts that will be used up before that date so we never have expired reagents around. That's easier than always having to inventory for expired reagents.
To justify your dating you can use published dates from various vendors (ie, datasheets, expirations on the bottle, etc).
For chemicals that do not state expiration dates I actually asked the question and got a written reply (via their website) from Fisher that states liquid chemicals are good for 3 years and dry chemicals for 5 years unless stated on the bottle. I put that letter in our manual as documentation on how we arrived at our in-house expiration dating.
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K.
Sent: Wednesday, December 10, 2014 8:45 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] shelf life
We are being inspected soon and I'm trying to check the labels on all of my chemicals. How do I determine the expiration date of a 1N HCl solution I prepared in house. I went on line and a commercially available 1N HCl solution has a 36 month shelf life according to the vendor's web site. I don't know how they arrived at that. I know concentrated HCl has an indefinite shelf life, but a 5 year retest date appears on the COA. It's confusing to me.
Any help would be appreciated.
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Message: 6
Date: Wed, 10 Dec 2014 17:00:49 +0000
From: "Hernandez, Jesus Willibaldo" <HernandezJW <@t> uthscsa.edu>
Subject: [Histonet] Solution Chemistry - Alcoholic Formalin - Bone
Histology - Fluorescent Label
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<histonet <@t> lists.utsouthwestern.edu>
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I was going to make a new Alcoholic Formalin Solution. Using the following recipe/ratio:
2700mL 95% EtOH
905mL DI H2O
95mL Formalin (37-40%)
Instead of using 37-40% Formalin, I am going to use a 10% buffered formalin solution containing the following chemicals/buffers:
Formaldehyde 36.5%, 10% V/V
Sodium Phosphate Monobasic, 0.4% W/V
Sodium Phosphate Dibasic, 0.65% W/V
Deionized Water <100% V/V
Since it is buffered already, I will be adding only 27mL of (10% Formalin) to 73mL (95% EtOH).
The solution is to be used to fix tetracycline florescent labels within bone tissue. My question is if this concentration will work and will the buffers interfere with the fixation procedure?
Regards,
Jesse H.
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Message: 7
Date: Wed, 10 Dec 2014 17:25:58 +0000 (UTC)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] shelf life
To: "Bitting, Angela K." <akbitting <@t> geisinger.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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Expiration dates and not only confusing but very variable and inaccurate. I do not think you should worry about how the vendor determined the exp. date???maybe it is just greater than a competitor's just to "lure" you to buy theirs, who knows, but you can??use their exp.date for yours.HCl absorbs water vapor??if the container??is not well capped, and that may be the "over-reacting" recommendation of testing is in 5 years, but it could be 4 years or 10 years depending in the environment humidity or the cap used.These regulations??are something like the "piggy banks", they make "parents bank robbers" and the exp.dates "make histotechs liers".With experience you will be able to determine that some solutions "weaken" after some time lapse, use those known lapses as your exp.date for those solutions and "expand" to others.The inspectors, in their "infinite wisdom" want to read a date, regarldess of its accuracy. Their??"ego" is saved by a written date.Ren?? J.??
On Wednesday, December 10, 2014 11:46 AM, "Bitting, Angela K." <akbitting <@t> geisinger.edu> wrote:
We are being inspected soon and I'm trying to check the labels on all of my chemicals.?? How do I determine the expiration date of a 1N HCl solution I prepared in house.?? I went on line and a commercially available 1N HCl solution has a 36 month shelf life according to the vendor's web site. I don't know how they arrived at that. I know concentrated HCl has an indefinite shelf life, but a 5 year retest date appears on the COA. It's confusing to me.
Any help would be appreciated.
IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________
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