[Histonet] Gomori Trichrome

[Lewis, Sarah]

Joanna, I agree with Tim... We also see this at times if the tissue has freezing artifact.  Gomori Trichrome is very unforgiving :)


Sarah E. Lewis HT, ASCP
The Research Institute at
Nationwide Children's Hospital
Center for Gene Therapy
Neuromuscular Division
Rm WA3110
(614)722-2204




-----Original Message-----
From: Morken, Timothy [mailto:Timothy.Morken <@t> ucsf.edu] 
Sent: Thursday, December 11, 2014 11:19 AM
To: 'Joanna'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Gomori Trichrome 

Joanna, If it looks like it is under the section, then I would suspect  trapped reagent in air bubbles, folds, etc. If it is on top of the section then maybe more rinsing is needed.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v2/url?u=http-3A__mailto-3Ahistonet-2Dbounces-40lists.utsouthwestern.edu&d=AAIFAg&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=7bt8tbqem5cRuX3hC3eOeQ&m=0vo4hfofYzOL04nyQnusz7sZCdl6SlMBYQxPhvKuLTo&s=9bOOBwtwMikwEx-4zwlWv08DLvU_t-ipNcKOpMi5GfA&e= ] On Behalf Of Joanna
Sent: Wednesday, December 10, 2014 9:19 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Gomori Trichrome 


Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. 

Hopefully  you can give us some suggestions. 

Thanks. 

Neuropathology lab
Vancouver, Canada 



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