[Histonet] RE: Gomori Trichrome

Thu Dec 11 12:32:46 CST 2014

Have you tried filtering the stain just before using it?
Cathy

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Subject: Histonet Digest, Vol 133, Issue 13

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Today's Topics:

   1. Gomori Trichrome  (Joanna)
   2. Lab power failures (Martin, Erin)
   3. IHC CONTROL LOG (Sidonie.Aten <@t> hcahealthcare.com)
   4. RE: Gomori Trichrome  (Morken, Timothy)

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Message: 1
Date: Wed, 10 Dec 2014 21:18:41 -0800
From: Joanna <jobaluyut <@t> gmail.com>
Subject: [Histonet] Gomori Trichrome
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
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Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. 

Hopefully  you can give us some suggestions. 

Thanks. 

Neuropathology lab
Vancouver, Canada 

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Message: 2
Date: Thu, 11 Dec 2014 10:37:37 +0000
From: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
Subject: [Histonet] Lab power failures
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Good morning all!  Would anyone mind sharing their plans for power failure?  We have backup generator power on red plugs but my pathologists want a plan for what we would do if the power went out AND the backup generator failed.

Thanks for your help!

Erin

Erin Martin, Histology Supervisor
UCSF  Dermatopathology Service
415-353-7248

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Message: 3
Date: Thu, 11 Dec 2014 16:15:00 +0000
From: <Sidonie.Aten <@t> hcahealthcare.com>
Subject: [Histonet] IHC CONTROL LOG
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BEEN DOING IHC FOR TWO YEARS.  WOULD LIKE TO KNOW HOW OTHERS ARE LOGGING THEIR CONTROL CASES.
THANKS,
SIDONIE

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Message: 4
Date: Thu, 11 Dec 2014 16:18:39 +0000
From: "Morken, Timothy" <Timothy.Morken <@t> ucsf.edu>
Subject: RE: [Histonet] Gomori Trichrome 
To: 'Joanna' <jobaluyut <@t> gmail.com>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
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Joanna, If it looks like it is under the section, then I would suspect  trapped reagent in air bubbles, folds, etc. If it is on top of the section then maybe more rinsing is needed.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanna
Sent: Wednesday, December 10, 2014 9:19 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Gomori Trichrome 

Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. 

Hopefully  you can give us some suggestions. 

Thanks. 

Neuropathology lab
Vancouver, Canada 

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