[Histonet] Solution Chemistry - Alcoholic Formalin - Bone Histology - Fluorescent Label

[Hernandez, Jesus Willibaldo]

I was going to make a new Alcoholic Formalin Solution. Using the following recipe/ratio:

2700mL 95% EtOH
905mL DI H2O
95mL Formalin (37-40%)

Instead of using 37-40% Formalin, I am going to use a 10% buffered formalin solution containing the following chemicals/buffers:

Formaldehyde 36.5%, 10% V/V
Sodium Phosphate Monobasic, 0.4% W/V
Sodium Phosphate Dibasic, 0.65% W/V
Deionized Water <100% V/V

Since it is buffered already, I will be adding only 27mL of (10% Formalin) to 73mL (95% EtOH).

The solution is to be used to fix tetracycline florescent labels within bone tissue. My question is if this concentration will work and will the buffers interfere with the fixation procedure?

Regards,

Jesse H.